Factors Promoting the Establishment of Primary Cultures of Liver Cells FromXenopus Larvae

Overview

Journal Wilehm Roux Arch Dev Biol

Specialty Anatomy & Histology

Date 2017 Mar 18

PMID 28305021

Citations 1

Authors

Walter Wahli

Rudolf Weber

Affiliations

Soon will be listed here.

Abstract

The requirements for establishment and survival of primary cultures of larval amphibian liver cells were investigated.Plating efficiency was found to be enhanced by a collagen substrate, by diluted conditioned medium from an adultXenopus kidney cell line and by high initial cell densities. Plating efficiency was highest at a tonicity of 165-220 mOsm/kg. In cultures with undiluted conditioned medium the increase in cell number was 50-60% greater than in controls, where it was about 2-fold between day 3 and 6 of culture. Conditioned medium from theXenopus kidney cell line is assumed to contain at least two components, which are effective at different concentrations and stimulate either plating efficiency and cell aggregation or cell proliferation.In cultures without collagen sheets, cell flattening is greatly reduced, indicating that cell shape is also dependent upon the substrate.

Citing Articles

Retention of the differentiated state by larvalXenopus liver cells in primary culture.

Wahli W, Abraham I, Weber R Wilehm Roux Arch Dev Biol. 2017; 185(3):235-248.

PMID: 28304990 DOI: 10.1007/BF00848354.

References

Solursh M, Reiter R . Long-term cell culture of two differentiated cell types from the liver of larval and adult Xenopus laevis. Z Zellforsch Mikrosk Anat. 1972; 128(4):457-69. DOI: 10.1007/BF00306982. View

Takahashi K, Okada T . Separation of two factors affecting the aggregation kinetics from the conditioned medium. Dev Growth Differ. 1971; 13(1):15-24. DOI: 10.1111/j.1440-169x.1971.00015.x. View

Shaffer B . The isolated Xenopus laevis tail: a preparation for studying the central nervous system and metamorphosis in culture. J Embryol Exp Morphol. 1963; 11:77-90. View

Wood G, KEECH M . The formation of fibrils from collagen solutions. 1. The effect of experimental conditions: kinetic and electron-microscope studies. Biochem J. 1960; 75:588-98. PMC: 1204515. DOI: 10.1042/bj0750588. View

Michalopoulos G, Pitot H . Primary culture of parenchymal liver cells on collagen membranes. Morphological and biochemical observations. Exp Cell Res. 1975; 94(1):70-8. DOI: 10.1016/0014-4827(75)90532-7. View

Chen P . Patterns and metamorphic changes of serum proteins in amphibia. Wilhelm Roux Arch Entwickl Mech Org. 2017; 165(2):132-149. DOI: 10.1007/BF00650142. View

Leffert H, Paul D . Studies on primary cultures of differentiated fetal liver cells. J Cell Biol. 1972; 52(3):559-68. PMC: 2108652. DOI: 10.1083/jcb.52.3.559. View

Koch K, Leffert H . Growth control of differentiated fetal rat hepatocytes in primary monolayer culture. VI. Studies with conditioned medium and its functional interactions with serum factors. J Cell Biol. 1974; 62(3):780-91. PMC: 2109209. DOI: 10.1083/jcb.62.3.780. View

Calderon J, UNANUE E . Two biological activities regulating cell proliferation found in cultures of peritoneal exudate cells. Nature. 1975; 253(5490):359-61. DOI: 10.1038/253359a0. View

10.

McGuire E . Intercellular adhesive selectivity. II. Properties of embryonic chick liver cell-cell adhesion. J Cell Biol. 1976; 68(1):90-100. PMC: 2109616. DOI: 10.1083/jcb.68.1.90. View

11.

Shambaugh 3rd G, BALINSKY J, Cohen P . Synthesis of carbamyl phosphate synthetase in amphibian liver in vitro. The effect of thyroxine. J Biol Chem. 1969; 244(19):5295-308. View

12.

GASCHE P . On the question of the point of attack of Thiouracil; Trials on Xenopus larvae. Experientia. 2010; 2:24-6. View

13.

Bennett T, Kriegstein H, Glenn J . Thyroxine stimulation of ornithine transcarbamylase activity and protein synthesis in tadpole (RNA catesbeiana) liver in organ culture. Biochem Biophys Res Commun. 1969; 34(4):412-7. DOI: 10.1016/0006-291x(69)90397-0. View

14.

Ledford B, Frieden E . Albumin synthesis during induced and spontaneous metamorphosis in the bullfrog Rana catesbeiana. Dev Biol. 1973; 30(1):187-97. DOI: 10.1016/0012-1606(73)90056-0. View

15.

Blatt L, Kim K, Cohen P . The effect of thyroxine on ribonucleic acid synthesis by premetamorphic tadpole liver cell suspensions. J Biol Chem. 1969; 244(18):4801-7. View

16.

Arthur E, Balls M . Amphibian cells in vitro. I. Growth of Xenopus cells in a soft agar medium and on an agar surface. Exp Cell Res. 1971; 64(1):113-8. DOI: 10.1016/0014-4827(71)90199-6. View

17.

Godsell P . The cell cycle of Xenopus laevis cells in monolayer culture. Exp Cell Res. 1974; 87(2):433-6. DOI: 10.1016/0014-4827(74)90512-6. View

18.

BALINSKY J, Coetzer T, Mattheyse F . The effect of thyroxine and hypertonic environment on the enzymes of the urea cycle in Xenopus laevis. Comp Biochem Physiol B. 1972; 43(1):83-95. DOI: 10.1016/0305-0491(72)90204-0. View

19.

Monnickendam M, Balls M . Amphibian organ culture. Experientia. 1973; 29(1):1-17. DOI: 10.1007/BF01913222. View

20.

Tata J . Requirement for RNA and protein synthesis for induced regression of the tadpole tail in organ culture. Dev Biol. 1966; 13(1):77-94. DOI: 10.1016/0012-1606(66)90050-9. View