Tentacle Morphogenesis in Hydra : I. The Morphological Effect of Actinomycin D

Overview

Journal Wilhelm Roux Arch Entwickl Mech Org

Specialty Anatomy & Histology

Date 2017 Mar 18

PMID 28304782

Citations 2

Authors

Georgia E Lesh-Laurie

Leminh Hang

Affiliations

Soon will be listed here.

Abstract

Hydra oligactis exposed to 3 μg/ml actinomycin D for 24 hours regenerated only the first pair of tentacles (the mid-laterals). If left uncut, actinomycin D treated animals underwent a reduction of the normal number of tentacles to two or less.Inductive activity was retained in the 2-tentacled hypostomes. However, the tentacles present exhibited reduced capacities to capture and manipulate prey.Histological studies showed that the tentacles of actinomycin D treated hydra were morphologically identical to those of the controls. The interstitial cell (I-cell) population of the treated animals, however, became depleted. Replacing the hypostome of an actinomycin D treated hydra with a normal hypostome reversed the cellular effects of actinomycin D treatment.The modifications in tentacle morphogenesis occurring after actinomycin D treatment are consistent with an impairment of hypostomal function in the animal. It is suggested that the morphological site of this malfunction may be in the nervous system.

Citing Articles

Biosynthetic events of hydrid regeneration : I. The role of DNA synthesis during tentacle elaboration.

Lesh-Laurie G, Brooks D, Kaplan E Wilehm Roux Arch Dev Biol. 2017; 180(3):157-174.

PMID: 28305520 DOI: 10.1007/BF00848572.

Evaluation of tentacle regeneration as a biological assay inHydra.

Flechtner V, Lesh-Laurie G, Abbott M Wilehm Roux Arch Dev Biol. 2017; 190(2):67-72.

PMID: 28305353 DOI: 10.1007/BF00848397.

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