[Inducing Capacity of Subcellular Fractions from the Gastrula OfXenopus Laevis]

Overview

Journal Wilhelm Roux Arch Entwickl Mech Org

Specialty Anatomy & Histology

Date 2017 Mar 18

PMID 28304569

Citations 7

Authors

Ilse Faulhaber

Affiliations

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Abstract

Isolated yolk platelets, nuclei and pigment granules, ribosomes and the ribosome-free supernatant of homogenates from whole gastrulae ofXenopus laevis were tested for their inducing properties.The subcellular fractions were obtained by differential centrifugation in sucrose media and their purity was checked by electronmicroscopy.The microsomal fraction shows archencephalic and deuterencephalic inducing capacity which is enhanced after extraction with pyrophosphate and with phenol. The membrane structures of this fraction can partially be separated from the ribosomes by treatment with desoxycholate. Proteins with deuterencephalic and spinocaudal inducing activity are extractable from the desoxycholate treated microsomal fraction with 1 M NHCl.The supernatant, free of ribosomes, has a rather weak inducing ability. After extraction with phenol the archencephalic and deuterencephalic activity increases, but not the spinoaudal.Isolated nuclei induce small deuterencephalic structures and a relatively high percentage of spinocaudal differentiations. Extraction with phenol does not enhance the rate of these inductions.The yolk platelets show mainly deuterencephalic inducing activity which is associated essentially with the superficial layer of these particles. Proteins, inducing archencephalic and deuterencephalic tissue differentiations, are extractable in small amounts from the platelets after partial removal of the superficial layer.

Citing Articles

Inducing activity of fractionated microsomal material from theXenopus laevis gastrula stage.

Wall R, Faulhaber I Wilehm Roux Arch Dev Biol. 2017; 180(3):207-212.

PMID: 28305523 DOI: 10.1007/BF00848575.

Neural-inducing activity of nuclei and nuclear fractions from Xenopus laevis embryos.

Bretzel G, Tiedemann H Rouxs Arch Dev Biol. 2017; 195(2):123-127.

PMID: 28305447 DOI: 10.1007/BF00456108.

[A comparison of the inducing ability from the superficial layer of the yolk platelet coats and the microsomal fraction of cleavage, gastrula and neurula stages of Xenopus laevis].

Faulhaber I, Lyra L Wilhelm Roux Arch Entwickl Mech Org. 2017; 176(2):151-157.

PMID: 28304839 DOI: 10.1007/BF02569024.

[Studies of the activity and the isoenzyme patterns of LDH during the development ofXenopus laevis, Triturus alpestris andvulgaris andAmbystoma mexicanum].

Faulhaber I, Lyra L Wilhelm Roux Arch Entwickl Mech Org. 2017; 176(3):191-205.

PMID: 28304825 DOI: 10.1007/BF00576801.

Mechanisms of cell differentiation: Affinity of a morphogenetic factor to DNA.

Tiedemann H, Born J, Tiedemann H Wilhelm Roux Arch Entwickl Mech Org. 2017; 171(2):160-169.

PMID: 28304572 DOI: 10.1007/BF00576334.

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