Redox-sensing Regulator Rex Regulates Aerobic Metabolism, Morphological Differentiation, and Avermectin Production in Streptomyces Avermitilis

Overview

Journal Sci Rep

Specialty Science

Date 2017 Mar 18

PMID 28303934

Citations 9

Authors

Xingchao Liu

Yaqing Cheng

Mengya Lyu

Ying Wen

Yuan Song

Zhi Chen

Jilun Li

Affiliations

Soon will be listed here.

Abstract

The regulatory role of redox-sensing regulator Rex was investigated in Streptomyces avermitilis. Eleven genes/operons were demonstrated to be directly regulated by Rex; these genes/operons are involved in aerobic metabolism, morphological differentiation, and secondary metabolism. Rex represses transcription of target genes/operons by binding to Rex operator (ROP) sequences in the promoter regions. NADH reduces DNA-binding activity of Rex to target promoters, while NAD competitively binds to Rex and modulates its DNA-binding activity. Rex plays an essential regulatory role in aerobic metabolism by controlling expression of the respiratory genes atpIBEFHAGDC, cydA1B1CD, nuoA1-N1, rex-hemAC1DB, hppA, and ndh2. Rex also regulates morphological differentiation by repressing expression of wblE, which encodes a putative WhiB-family transcriptional regulator. A rex-deletion mutant (Drex) showed higher avermectin production than the wild-type strain ATCC31267, and was more tolerant of oxygen limitation conditions in regard to avermectin production.

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