Novel Device for Application of Continuous Mechanical Tensile Strain to Mammalian Cells

Overview

Journal Biol Open

Specialty Biology

Date 2017 Mar 18

PMID 28302667

Citations 6

Authors

Satoshi Wada

Hiroyuki Kanzaki

Tsuyoshi Narimiya

Yoshiki Nakamura

Affiliations

Soon will be listed here.

Abstract

During orthodontic tooth movement, the periodontal ligament (PDL) is exposed to continuous mechanical strain. However, many researchers have applied cyclic tensile strain, not continuous tensile strain, to PDL cells because there has been no adequate device to apply continuous tensile strain to cultured cells. In this study, we contrived a novel device designed to apply continuous tensile strain to cells in culture. The continuous tensile strain was applied to human immortalized periodontal ligament cell line (HPL cells) and the cytoskeletal structures of HPL cells were examined by immunohistochemistry. The expression of both inflammatory and osteogenic markers was also examined by real-time reverse transcription polymerase chain reaction. The osteogenic protein, Osteopontin (OPN), was also detected by western blot analysis. The actin filaments of HPL cells showed uniform arrangement under continuous tensile strain. The continuous tensile strain increased the expression of inflammatory genes such as , , and , and osteogenic genes such as and in HPL cells. It also elevated the expression of OPN protein in HPL cells. These results suggest that our new simple device is useful for exploring the responses to continuous tensile strain applied to the cells.

Citing Articles

Effect of Mechanical Force Stress on the Inflammatory Response in Human Periodontal Ligament Cells.

Rojasawasthien T, Srithanyarat S, Bulanawichit W, Osathanon T Int Dent J. 2024; 75(1):117-126.

PMID: 39730290 PMC: 11806315. DOI: 10.1016/j.identj.2024.12.001.

Effect of Different Parameters of In Vitro Static Tensile Strain on Human Periodontal Ligament Cells Simulating the Tension Side of Orthodontic Tooth Movement.

Sun C, Janjic Rankovic M, Folwaczny M, Stocker T, Otto S, Wichelhaus A Int J Mol Sci. 2022; 23(3).

PMID: 35163446 PMC: 8835937. DOI: 10.3390/ijms23031525.

The Phenotypic Responses of Vascular Smooth Muscle Cells Exposed to Mechanical Cues.

Jensen L, Bentzon J, Albarran-Juarez J Cells. 2021; 10(9).

PMID: 34571858 PMC: 8469800. DOI: 10.3390/cells10092209.

Effect of Tension on Human Periodontal Ligament Cells: Systematic Review and Network Analysis.

Sun C, Janjic Rankovic M, Folwaczny M, Otto S, Wichelhaus A, Baumert U Front Bioeng Biotechnol. 2021; 9:695053.

PMID: 34513810 PMC: 8429507. DOI: 10.3389/fbioe.2021.695053.

A Novel Tension Machine Promotes Bone Marrow Mesenchymal Stem Cell Osteoblastic and Fibroblastic Differentiation by Applying Cyclic Tension.

Zhao Y, Huang Y, Jia L, Wang R, Tan K, Li W Stem Cells Int. 2021; 2021:6647651.

PMID: 34422062 PMC: 8371653. DOI: 10.1155/2021/6647651.

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