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Common Organization of Gene Clusters for Production of Different Capsular Polysaccharides (K Antigens) in Escherichia Coli

Overview
Journal J Bacteriol
Publisher American Society for Microbiology
Specialty Microbiology
Date 1988 Mar 1
PMID 2830235
Citations 48
Authors
I S Roberts
R Mountford
R Hodge
K B Jann
G J Boulnois
Affiliations
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Abstract

Southern blot analysis of cloned K5- and K7-antigen genes, using DNA fragments from cloned K1 genes as radiolabeled probes, demonstrated that each K-antigen gene cluster is organized in a manner similar to that shown for the K1 antigen. That is, a central DNA segment unique for a given antigen type is flanked by DNA sequences that encode common functions for the management of intracellular polymer. This has been confirmed by transposon and deletion mutagenesis of plasmids carrying the K5 and K7 genes. We also describe a series of complementation experiments in which transport or postpolymerizational modification functions for one K antigen are used to complement mutations in the corresponding regions of a different K-antigen gene cluster. Thus, postpolymerizational modification of polysaccharide and transport of mature polysaccharide from the periplasmic space are common mechanisms and are independent of polysaccharide structure.

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References
1.
Clewell D, Helinski D . Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969; 62(4):1159-66. PMC: 223628. DOI: 10.1073/pnas.62.4.1159. View
2.
Frosch M, Roberts I, Gorgen I, Metzger S, Boulnois G, Bitter-Suermann D . Serotyping and genotyping of encapsulated Escherichia coli K1 sepsis isolates with a monoclonal IgG anti K1 antibody and K1 gene probes. Microb Pathog. 1987; 2(5):319-26. DOI: 10.1016/0882-4010(87)90074-x. View
3.
ORSKOV I, ORSKOV F, JANN B, Jann K . Serology, chemistry, and genetics of O and K antigens of Escherichia coli. Bacteriol Rev. 1977; 41(3):667-710. PMC: 414020. DOI: 10.1128/br.41.3.667-710.1977. View
4.
Birnboim H, DOLY J . A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979; 7(6):1513-23. PMC: 342324. DOI: 10.1093/nar/7.6.1513. View
5.
Gotschlich E, Fraser B, Nishimura O, Robbins J, Liu T . Lipid on capsular polysaccharides of gram-negative bacteria. J Biol Chem. 1981; 256(17):8915-21. View