White Matter Repair After Extracellular Vesicles Administration in an Experimental Animal Model of Subcortical Stroke

Overview

Journal Sci Rep

Specialty Science

Date 2017 Mar 17

PMID 28300134

Citations 114

Authors

Laura Otero-Ortega

Fernando Laso-Garcia

Maria Del Carmen Gomez-de Frutos

Berta Rodriguez-Frutos

Jorge Pascual-Guerra

Blanca Fuentes

Exuperio Diez-Tejedor

Maria Gutierrez-Fernandez

Affiliations

Soon will be listed here.

Abstract

Mesenchymal stem cells have previously been shown to mediate brain repair after stroke; they secrete 50-100 nm complexes called extracellular vesicles (EVs), which could be responsible for provoking neurovascular repair and functional recovery. EVs have been observed by electron microscopy and NanoSight, and they contain associated proteins such as CD81 and Alix. This purified, homogeneous population of EVs was administered intravenously after subcortical stroke in rats. To evaluate the EVs effects, we studied the biodistribution, proteomics analysis, functional evaluation, lesion size, fiber tract integrity, axonal sprouting and white matter repair markers. We found that a single administration of EVs improved functional recovery, fiber tract integrity, axonal sprouting and white matter repair markers in an experimental animal model of subcortical stroke. EVs were found in the animals' brain and peripheral organs after euthanasia. White matter integrity was in part restored by EVs administration mediated by molecular repair factors implicated in axonal sprouting, tract connectivity, remyelination and oligodendrogenesis. These findings are associated with improved functional recovery. This novel role for EVs presents a new perspective in the development of biologics for brain repair.

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