Addressing Challenges to Enhance the Bioactives of Through Organ, Tissue, and Cell Culture Based Approaches

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2017 Mar 17

PMID 28299323

Citations 5

Authors

Pritika Singh

Rupam Guleri

Amrita Angurala

Kuldeep Kaur

Kulwinder Kaur

Sunil C Kaul

Renu Wadhwa

Pratap Kumar Pati

Affiliations

Soon will be listed here.

Abstract

is a highly valued medicinal plant in traditional home medicine and is known for a wide range of bioactivities. Its commercial cultivation is adversely affected by poor seed viability and germination. Infestation by various pests and pathogens, survival under unfavourable environmental conditions, narrow genetic base, and meager information regarding biosynthesis of secondary metabolites are some of the other existing challenges in the crop. Biotechnological interventions through organ, tissue, and cell culture provide promising options for addressing some of these issues. propagation facilitates conservation and sustainable utilization of the existing germplasms and broadening the genetic base. It would also provide means for efficient and rapid mass propagation of elite chemotypes and generating uniform plant material round the year for experimentation and industrial applications. The potential of cell/organ cultures for the production of therapeutically valuable compounds and their large-scale production in bioreactors has received significant attention in recent years. culture system further provides distinct advantage for studying various cellular and molecular processes leading to secondary metabolite accumulation and their regulation. Engineering plants through genetic transformation and development of hairy root culture system are powerful strategies for modulation of secondary metabolites. The present review highlights the developments and sketches current scenario in this field.

Citing Articles

Biotechnological interventions in banana: current knowledge and future prospects.

Justine A, Kaur N, Savita , Pati P Heliyon. 2022; 8(11):e11636.

PMID: 36419664 PMC: 9676558. DOI: 10.1016/j.heliyon.2022.e11636.

Optimization of Elicitation Conditions to Enhance the Production of Potent Metabolite Withanolide from (L.).

Singh M, Agrawal S, Afzal O, Altamimi A, Redhwan A, Alshammari N Metabolites. 2022; 12(9).

PMID: 36144259 PMC: 9502510. DOI: 10.3390/metabo12090854.

Critical factors influencing in vitro propagation and modulation of important secondary metabolites in (L.) dunal.

Kaur K, Dolker D, Behera S, Pati P Plant Cell Tissue Organ Cult. 2022; 149(1-2):41-60.

PMID: 35039702 PMC: 8754361. DOI: 10.1007/s11240-021-02225-w.

Innate endophytic fungus, Aspergillus terreus as biotic elicitor of withanolide A in root cell suspension cultures of Withania somnifera.

Kumar Kushwaha R, Singh S, Pandey S, Kalra A, Vivek Babu C Mol Biol Rep. 2019; 46(2):1895-1908.

PMID: 30706360 DOI: 10.1007/s11033-019-04641-w.

: Advances and Implementation of Molecular and Tissue Culture Techniques to Enhance Its Application.

Pandey V, Ansari W, Misra P, Atri N Front Plant Sci. 2017; 8:1390.

PMID: 28848589 PMC: 5552756. DOI: 10.3389/fpls.2017.01390.

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