Fingerprint Analysis and Identification of Strains ST309 As a Potential High Risk Clone in a Population Isolated from Children with Bacteremia in Mexico City

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2017 Mar 17

PMID 28298909

Citations 15

Authors

Rosario Morales-Espinosa

Gabriela Delgado

Luis F Espinosa

Dassaev Isselo

Jose L Mendez

Cristina Rodriguez

Guadalupe Miranda

Alejandro Cravioto

Affiliations

Soon will be listed here.

Abstract

is an opportunistic pathogen and is associated with nosocomial infections. Its ability to thrive in a broad range of environments is due to a large and diverse genome of which its accessory genome is part. The objective of this study was to characterize strains isolated from children who developed bacteremia, using pulse-field gel electrophoresis, and in terms of its genomic islands, virulence genes, multilocus sequence type, and antimicrobial susceptibility. Our results showed that strains presented the seven virulence genes: 1, and , a type IV pilin alleles (TFP) group I or II. Additionally, we detected a novel pilin and accessory gene, expanding the number of TFP alleles to group VI. All strains presented the PAPI-2 Island and the majority were + and + genotype. Ten percent of the strains were multi-drug resistant phenotype, 18% extensively drug-resistant, 68% moderately resistant and only 3% were susceptible to all the antimicrobial tested. The most prevalent acquired β-Lactamase was KPC. We identified a group of ST309 strains, as a potential high risk clone. Our finding also showed that the strains isolated from patients with bacteremia have important virulence factors involved in colonization and dissemination as: a TFP group I or II; the presence of the gene within the PAPI-2 island and the presence of the gene.

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References

Rybtke M, Hultqvist L, Givskov M, Tolker-Nielsen T . Pseudomonas aeruginosa Biofilm Infections: Community Structure, Antimicrobial Tolerance and Immune Response. J Mol Biol. 2015; 427(23):3628-45. DOI: 10.1016/j.jmb.2015.08.016. View

Engel J, Balachandran P . Role of Pseudomonas aeruginosa type III effectors in disease. Curr Opin Microbiol. 2009; 12(1):61-6. DOI: 10.1016/j.mib.2008.12.007. View

Cisz M, Lee P, Rietsch A . ExoS controls the cell contact-mediated switch to effector secretion in Pseudomonas aeruginosa. J Bacteriol. 2007; 190(8):2726-38. PMC: 2293250. DOI: 10.1128/JB.01553-07. View

Thuong M, Arvaniti K, Ruimy R, de la Salmoniere P, Scanvic-Hameg A, Lucet J . Epidemiology of Pseudomonas aeruginosa and risk factors for carriage acquisition in an intensive care unit. J Hosp Infect. 2003; 53(4):274-82. DOI: 10.1053/jhin.2002.1370. View

Schmidt K, Tummler B, Romling U . Comparative genome mapping of Pseudomonas aeruginosa PAO with P. aeruginosa C, which belongs to a major clone in cystic fibrosis patients and aquatic habitats. J Bacteriol. 1996; 178(1):85-93. PMC: 177624. DOI: 10.1128/jb.178.1.85-93.1996. View

Grisaru-Soen G, Lerner-Geva L, Keller N, Berger H, Passwell J, Barzilai A . Pseudomonas aeruginosa bacteremia in children: analysis of trends in prevalence, antibiotic resistance and prognostic factors. Pediatr Infect Dis J. 2000; 19(10):959-63. DOI: 10.1097/00006454-200010000-00003. View

Liang X, Pham X, Olson M, Lory S . Identification of a genomic island present in the majority of pathogenic isolates of Pseudomonas aeruginosa. J Bacteriol. 2001; 183(3):843-53. PMC: 94950. DOI: 10.1128/JB.183.3.843-853.2001. View

Rumbaugh K, Hamood A, Griswold J . Analysis of Pseudomonas aeruginosa clinical isolates for possible variations within the virulence genes exotoxin A and exoenzyme S. J Surg Res. 1999; 82(1):95-105. DOI: 10.1006/jsre.1998.5523. View

Agodi A, Barchitta M, Cipresso R, Giaquinta L, Romeo M, Denaro C . Pseudomonas aeruginosa carriage, colonization, and infection in ICU patients. Intensive Care Med. 2007; 33(7):1155-1161. DOI: 10.1007/s00134-007-0671-6. View

10.

Mena K, Gerba C . Risk assessment of Pseudomonas aeruginosa in water. Rev Environ Contam Toxicol. 2009; 201:71-115. DOI: 10.1007/978-1-4419-0032-6_3. View

11.

Leone I, Chirillo M, Raso T, Zucca M, Savoia D . Phenotypic and genotypic characterization of Pseudomonas aeruginosa from cystic fibrosis patients. Eur J Clin Microbiol Infect Dis. 2008; 27(11):1093-9. DOI: 10.1007/s10096-008-0551-1. View

12.

Fergie J, Shema S, Lott L, Crawford R, Patrick C . Pseudomonas aeruginosa bacteremia in immunocompromised children: analysis of factors associated with a poor outcome. Clin Infect Dis. 1994; 18(3):390-4. DOI: 10.1093/clinids/18.3.390. View

13.

Poole K . Pseudomonas aeruginosa: resistance to the max. Front Microbiol. 2011; 2:65. PMC: 3128976. DOI: 10.3389/fmicb.2011.00065. View

14.

Wiehlmann L, Wagner G, Cramer N, Siebert B, Gudowius P, Morales G . Population structure of Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2007; 104(19):8101-6. PMC: 1876578. DOI: 10.1073/pnas.0609213104. View

15.

Hacker J, Kaper J . Pathogenicity islands and the evolution of microbes. Annu Rev Microbiol. 2000; 54:641-79. DOI: 10.1146/annurev.micro.54.1.641. View

16.

Morita Y, Tomida J, Kawamura Y . Efflux-mediated fluoroquinolone resistance in the multidrug-resistant Pseudomonas aeruginosa clinical isolate PA7: identification of a novel MexS variant involved in upregulation of the mexEF-oprN multidrug efflux operon. Front Microbiol. 2015; 6:8. PMC: 4301020. DOI: 10.3389/fmicb.2015.00008. View

17.

Sun Y, Karmakar M, Taylor P, Rietsch A, Pearlman E . ExoS and ExoT ADP ribosyltransferase activities mediate Pseudomonas aeruginosa keratitis by promoting neutrophil apoptosis and bacterial survival. J Immunol. 2012; 188(4):1884-95. PMC: 3273577. DOI: 10.4049/jimmunol.1102148. View

18.

Woodford N, Turton J, Livermore D . Multiresistant Gram-negative bacteria: the role of high-risk clones in the dissemination of antibiotic resistance. FEMS Microbiol Rev. 2011; 35(5):736-55. DOI: 10.1111/j.1574-6976.2011.00268.x. View

19.

Holloway B . Genetic recombination in Pseudomonas aeruginosa. J Gen Microbiol. 1955; 13(3):572-81. DOI: 10.1099/00221287-13-3-572. View

20.

Dobrindt U, Hochhut B, Hentschel U, Hacker J . Genomic islands in pathogenic and environmental microorganisms. Nat Rev Microbiol. 2004; 2(5):414-24. DOI: 10.1038/nrmicro884. View