Modulation of Cytochrome Oxidase Activity by Inorganic and Organic Phosphate

Overview

Journal Biochem J

Specialty Biochemistry

Date 1987 Nov 15

PMID 2829818

Citations 6

Authors

F Malatesta

G Antonini

P Sarti

M Brunori

Affiliations

Soon will be listed here.

Abstract

The activity of cytochrome oxidase reconstituted into phospholipid vesicles has been studied as a function of orthophosphate, ATP and inositol hexakisphosphate concentrations. The respiratory-control ratio was found to be quite sensitive to these compounds and was inversely related to the anion concentration. This effect is related to a phosphate-dependent decrease in the rate constant for ferrocytochrome c oxidation observed in the presence of ionophores. The data cannot be interpreted simply on the basis of ionic strength, which is known to limit cytochrome c binding to cytochrome oxidase, since cytochrome oxidase-containing vesicles responded differently to phosphate depending on the energization state of the phospholipid membrane.

Citing Articles

Tissue-specific regulation of bovine heart cytochrome-c oxidase activity by ADP via interaction with subunit VIa.

Anthony G, Reimann A, KADENBACH B Proc Natl Acad Sci U S A. 1993; 90(5):1652-6.

PMID: 8383320 PMC: 45937. DOI: 10.1073/pnas.90.5.1652.

ATP-induced spectral changes in cytochrome c oxidase. A kinetic investigation.

Antonini G, Malatesta F, Sarti P, Vallone B, Brunori M Biochem J. 1988; 256(3):835-40.

PMID: 2852006 PMC: 1135491. DOI: 10.1042/bj2560835.

Anions induce conformational changes and influence the activity and photoaffinity-labelling by 8-azido-ATP of isolated cytochrome c oxidase.

Reimann A, Huther F, Berden J, KADENBACH B Biochem J. 1988; 254(3):723-30.

PMID: 2848497 PMC: 1135144. DOI: 10.1042/bj2540723.

Inhibition of the phosphate-stimulated cytochrome c oxidase activity by thiophosphate.

Manon S, Camougrand N, Guerin M J Bioenerg Biomembr. 1989; 21(3):387-401.

PMID: 2545671 DOI: 10.1007/BF00762729.

Effect of gangliosides on membrane permeability studied by enzymic and fluorescence-spectroscopy techniques.

Sarti P, Antonini G, Malatesta F, Vallone B, Brunori M, Masserini M Biochem J. 1990; 267(2):413-6.

PMID: 2159281 PMC: 1131304. DOI: 10.1042/bj2670413.

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