Production of Cytopathology in FRhK-4 Cells by BS-C-1-passaged Hepatitis A Virus

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1987 Dec 1

PMID 2829721

Citations 11

Authors

A M Nasser

T G Metcalf

Affiliations

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Abstract

Cytopathic effects were produced in fetal rhesus monkey kidney (FRhK-4) cells 7 days postinfection by a serially BS-C-1-passaged strain of hepatitis A virus. Typical enterovirus cytopathology was produced by the HM-175 strain after 15 passages at 7-day intervals in BS-C-1 cells. No cytopathic effects were obtained after neutralization of virus with human anti-hepatitis A virus immunoglobulin G. Normal human serum had no effect on development of cytopathology. Maximum antigen and cDNA probe-based hybridization activity were associated with a CsCl gradient fraction having a density of 1.34 g/cm3. Large quantities of 27- to 30-nm virions typical of hepatitis A virus were associated with the same fraction. These data led to the conclusion that the observed cytopathology was caused by hepatitis A virus.

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References

Plagemann P, SWIM H . Replication of mengovirus. I. Effect on synthesis of macromolecules by host cell. J Bacteriol. 1966; 91(6):2317-26. PMC: 316213. DOI: 10.1128/jb.91.6.2317-2326.1966. View

Smith K, Melnick J . Electron microscopic counting of virus particles by sedimentation on aluminized grids. J Immunol. 1962; 89:279-84. View

Hollinger F, Bradley D, MAYNARD J, Dreesman G, Melnick J . Detection of hepatitis A viral antigen by radioimmunoassay. J Immunol. 1975; 115(5):1464-6. View

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Provost P, HILLEMAN M . Propagation of human hepatitis A virus in cell culture in vitro. Proc Soc Exp Biol Med. 1979; 160(2):213-21. DOI: 10.3181/00379727-160-40422. View

Frosner G, Deinhardt F, Scheid R, Gauss-Muller V, Holmes N, Messelberger V . Propagation of human hepatitis A virus in a hepatoma cell line. Infection. 1979; 7(6):303-5. DOI: 10.1007/BF01642154. View

Flehmig B . Hepatitis A-virus in cell culture: I. propagation of different hepatitis A-virus isolates in a fetal rhesus monkey kidney cell line (Frhk-4). Med Microbiol Immunol. 1980; 168(4):239-48. DOI: 10.1007/BF02121807. View

Daemer R, Feinstone S, Gust I, Purcell R . Propagation of human hepatitis A virus in African green monkey kidney cell culture: primary isolation and serial passage. Infect Immun. 1981; 32(1):388-93. PMC: 350632. DOI: 10.1128/iai.32.1.388-393.1981. View

Gauss-Muller V, Frosner G, Deinhardt F . Propagation of hepatitis A virus in human embryo fibroblasts. J Med Virol. 1981; 7(3):233-9. DOI: 10.1002/jmv.1890070308. View

10.

Salacinski P, Mclean C, Sykes J, Lowry P . Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenyl glycoluril (Iodogen). Anal Biochem. 1981; 117(1):136-46. DOI: 10.1016/0003-2697(81)90703-x. View

11.

Emini E, Jameson B, Lewis A, Larsen G, Wimmer E . Poliovirus neutralization epitopes: analysis and localization with neutralizing monoclonal antibodies. J Virol. 1982; 43(3):997-1005. PMC: 256210. DOI: 10.1128/JVI.43.3.997-1005.1982. View

12.

Parry J, Richmond J, Gardner S . Polyomavirus in fetal rhesus monkey kidney cell lines used to grow hepatitis A virus. Lancet. 1983; 1(8331):994. DOI: 10.1016/s0140-6736(83)92121-9. View

13.

Lemon S, Binn L, MARCHWICKI R . Radioimmunofocus assay for quantitation of hepatitis A virus in cell cultures. J Clin Microbiol. 1983; 17(5):834-9. PMC: 272750. DOI: 10.1128/jcm.17.5.834-839.1983. View

14.

Simmonds R, Szucs G, Metcalf T, Melnick J . Persistently infected cultures as a source of hepatitis A virus. Appl Environ Microbiol. 1985; 49(4):749-55. PMC: 238439. DOI: 10.1128/aem.49.4.749-755.1985. View

15.

Venuti A, Di Russo C, Grosso N, Patti A, Ruggeri F, De Stasio P . Isolation and molecular cloning of a fast-growing strain of human hepatitis A virus from its double-stranded replicative form. J Virol. 1985; 56(2):579-88. PMC: 252615. DOI: 10.1128/JVI.56.2.579-588.1985. View

16.

Wheeler C, Fields H, Schable C, Meinke W, MAYNARD J . Adsorption, purification, and growth characteristics of hepatitis A virus strain HAS-15 propagated in fetal rhesus monkey kidney cells. J Clin Microbiol. 1986; 23(3):434-40. PMC: 268669. DOI: 10.1128/jcm.23.3.434-440.1986. View

17.

Jiang X, Estes M, Metcalf T, Melnick J . Detection of hepatitis A virus in seeded estuarine samples by hybridization with cDNA probes. Appl Environ Microbiol. 1986; 52(4):711-7. PMC: 239102. DOI: 10.1128/aem.52.4.711-717.1986. View

18.

Anderson D . Cytopathology, plaque assay, and heat inactivation of hepatitis A virus strain HM175. J Med Virol. 1987; 22(1):35-44. DOI: 10.1002/jmv.1890220106. View

19.

Cromeans T, Sobsey M, Fields H . Development of a plaque assay for a cytopathic, rapidly replicating isolate of hepatitis A virus. J Med Virol. 1987; 22(1):45-56. DOI: 10.1002/jmv.1890220107. View

20.

Nasser A, Metcalf T . An A-ELISA to detect hepatitis A virus in estuarine samples. Appl Environ Microbiol. 1987; 53(5):1192-5. PMC: 203834. DOI: 10.1128/aem.53.5.1192-1195.1987. View