Cytotoxic and Apoptotic Effects of Different Extracts of Willd. on K562 and HL-60 Cell Lines

Overview

Journal Iran J Basic Med Sci

Publisher Mashhad University of Medical Sciences

Specialty General Medicine

Date 2017 Mar 16

PMID 28293393

Citations 15

Authors

Zahra Tayarani-Najaran

Farideh-Sadat Makki

Nafiseh-Sadat Alamolhodaei

Mahdi Mojarrab

Seyed Ahmad Emami

Affiliations

Soon will be listed here.

Abstract

Objectives: Artemisia is a genus of herbs and small shrubs forms an important part of natural vegetation in Iran. It has been reported that several Artemisia species possess anti-proliferative effects. Considering the value of this genus in anti-cancer researches we have chosen Artemisia biennis for cytotoxic and mechanistic studies.

Materials And Methods: In this study we have investigated the cytotoxic and apoptotic effects of petroleum ether, dichloromethane, ethyl acetate, ethanol, and ethanol: water (1:1 v/v) extracts of A. biennis Willd. on two cancer human cell lines (K562 and HL-60) and J774 as normal cells.

Results: CHCl extract was found to have the highest anti-proliferative effect on cancer cells. IC values obtained in AlamarBlue assay for CHCl extract were 64.86 and 54.31 µg/ml on K562 and HL-60 cells respectively. In flow cytometry histogram of the cells treated with CHCl extract, sub-G1 peak was induced. DNA fragmentation, increased in the level of Bax and cleavage of PARP protein all showed the induction of apoptosis with CHCl extract after 48 hr contact with cells.

Conclusion: The results can corroborate the cytotoxic and apoptotic effects of the CHCl extract of A. biennis on the K562 and HL-60 cancer cell lines.

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