Synthetic Peptides Derived from Bovine Lactoferricin Exhibit Antimicrobial Activity Against E. Coli ATCC 11775, S. Maltophilia ATCC 13636 and S. Enteritidis ATCC 13076

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2017 Mar 14

PMID 28287494

Citations 23

Authors

Nataly De Jesus Huertas Mendez

Yerly Vargas Casanova

Anyelith Katherine Gomez Chimbi

Edith Hernandez

Aura Lucia Leal Castro

Javier Mauricio Melo Diaz

Zuly Jenny Rivera Monroy

Javier Eduardo Garcia Castaneda

Affiliations

Soon will be listed here.

Abstract

Linear, dimeric, tetrameric, and cyclic peptides derived from lactoferricin B-containing non-natural amino acids and the RWQWR motif were synthesized, purified, and characterized using RP-HPLC, MALDI-TOF mass spectrometry, and circular dichroism. The antibacterial activity of peptides against ATCC 11775, ATCC 13636, and ATCC 13076 was evaluated. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. The synthetic bovine lactoferricin exhibited antibacterial activity against ATCC 11775 and ATCC 13076. The dimeric peptide (RRWQWR)₂K-Ahx exhibited the highest antibacterial activity against the tested bacterial strain. The monomeric, cyclic, tetrameric, and palindromic peptides containing the RWQWR motif exhibited high and specific activity against ATCC 11775. The results suggest that short peptides derived from lactoferricin B could be considered as potential candidates for the development of antibacterial agents against infections caused by .

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References

Garcia J, Fierro R, Puentes A, Cortes J, Bermudez A, Cifuentes G . Monosaccharides modulate HCV E2 protein-derived peptide biological properties. Biochem Biophys Res Commun. 2007; 355(2):409-18. DOI: 10.1016/j.bbrc.2007.01.167. View

Garcia-Montoya I, Cendon T, Arevalo-Gallegos S, Rascon-Cruz Q . Lactoferrin a multiple bioactive protein: an overview. Biochim Biophys Acta. 2011; 1820(3):226-36. PMC: 7127262. DOI: 10.1016/j.bbagen.2011.06.018. View

Tu Y, Ho Y, Chuang Y, Chen P, Chen C . Identification of lactoferricin B intracellular targets using an Escherichia coli proteome chip. PLoS One. 2011; 6(12):e28197. PMC: 3229523. DOI: 10.1371/journal.pone.0028197. View

Brooke J . Stenotrophomonas maltophilia: an emerging global opportunistic pathogen. Clin Microbiol Rev. 2012; 25(1):2-41. PMC: 3255966. DOI: 10.1128/CMR.00019-11. View

Qamruddin A, Alkawash M, Soothill J . Antibiotic susceptibility of Stenotrophomonas maltophilia in the presence of lactoferrin. Antimicrob Agents Chemother. 2005; 49(10):4425-6. PMC: 1251558. DOI: 10.1128/AAC.49.10.4425-4426.2005. View

Ghazaey S, Mirmomeni M . Microbial-resistant Salmonella enteritidis isolated from poultry samples. Rep Biochem Mol Biol. 2016; 1(1):9-13. PMC: 4757074. View

Fang B, Guo H, Zhang M, Jiang L, Ren F . The six amino acid antimicrobial peptide bLFcin6 penetrates cells and delivers siRNA. FEBS J. 2012; 280(4):1007-17. DOI: 10.1111/febs.12093. View

Solarte V, Rosas J, Rivera Z, Arango-Rodriguez M, Garcia J, Vernot J . A Tetrameric Peptide Derived from Bovine Lactoferricin Exhibits Specific Cytotoxic Effects against Oral Squamous-Cell Carcinoma Cell Lines. Biomed Res Int. 2015; 2015:630179. PMC: 4644816. DOI: 10.1155/2015/630179. View

Sinha M, Kaushik S, Kaur P, Sharma S, Singh T . Antimicrobial lactoferrin peptides: the hidden players in the protective function of a multifunctional protein. Int J Pept. 2013; 2013:390230. PMC: 3608178. DOI: 10.1155/2013/390230. View

10.

Ulvatne H, Samuelsen O, Haukland H, Kramer M, Vorland L . Lactoferricin B inhibits bacterial macromolecular synthesis in Escherichia coli and Bacillus subtilis. FEMS Microbiol Lett. 2004; 237(2):377-84. DOI: 10.1016/j.femsle.2004.07.001. View

11.

Oo T, Cole N, Garthwaite L, Willcox M, Zhu H . Evaluation of synergistic activity of bovine lactoferricin with antibiotics in corneal infection. J Antimicrob Chemother. 2010; 65(6):1243-51. DOI: 10.1093/jac/dkq106. View

12.

Greathouse D, Vostrikov V, McClellan N, Chipollini J, Lay J, Liyanage R . Lipid interactions of acylated tryptophan-methylated lactoferricin peptides by solid-state NMR. J Pept Sci. 2008; 14(10):1103-10. DOI: 10.1002/psc.1047. View

13.

Al-Jasser A . Stenotrophomonas maltophilia resistant to trimethoprim-sulfamethoxazole: an increasing problem. Ann Clin Microbiol Antimicrob. 2006; 5:23. PMC: 1578578. DOI: 10.1186/1476-0711-5-23. View

14.

Wakabayashi H, Matsumoto H, Hashimoto K, Teraguchi S, Takase M, Hayasawa H . N-Acylated and D enantiomer derivatives of a nonamer core peptide of lactoferricin B showing improved antimicrobial activity. Antimicrob Agents Chemother. 1999; 43(5):1267-9. PMC: 89256. DOI: 10.1128/AAC.43.5.1267. View

15.

Bellamy W, Takase M, Yamauchi K, Wakabayashi H, Kawase K, Tomita M . Identification of the bactericidal domain of lactoferrin. Biochim Biophys Acta. 1992; 1121(1-2):130-6. DOI: 10.1016/0167-4838(92)90346-f. View

16.

Chan D, Prenner E, Vogel H . Tryptophan- and arginine-rich antimicrobial peptides: structures and mechanisms of action. Biochim Biophys Acta. 2006; 1758(9):1184-202. DOI: 10.1016/j.bbamem.2006.04.006. View

17.

Vorland L, Ulvatne H, Andersen J, Haukland H, Rekdal O, Svendsen J . Antibacterial effects of lactoferricin B. Scand J Infect Dis. 1999; 31(2):179-84. DOI: 10.1080/003655499750006245. View

18.

Liu Y, Han F, Xie Y, Wang Y . Comparative antimicrobial activity and mechanism of action of bovine lactoferricin-derived synthetic peptides. Biometals. 2011; 24(6):1069-78. DOI: 10.1007/s10534-011-9465-y. View

19.

Brouwer C, Rahman M, Welling M . Discovery and development of a synthetic peptide derived from lactoferrin for clinical use. Peptides. 2011; 32(9):1953-63. DOI: 10.1016/j.peptides.2011.07.017. View

20.

Branen J, Davidson P . Activity of hydrolysed lactoferrin against foodborne pathogenic bacteria in growth media: the effect of EDTA. Lett Appl Microbiol. 2000; 30(3):233-7. DOI: 10.1046/j.1472-765x.2000.00711.x. View