Unusual DNA Structure in the Regulatory Region of the Human Papovavirus JC Virus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1988 Mar 1

PMID 2828687

Citations 7

Authors

S Amirhaeri

F WOHLRAB

E O Major

R D Wells

Affiliations

Soon will be listed here.

Abstract

The human papovavirus JC virus (JCV) was analyzed for the presence of unusual DNA conformations. Recombinant plasmids containing 60% of the JCV prototype Mad-1 strain DNA were constructed and analyzed with both enzymatic and chemical probes. Fine-mapping studies revealed that the most prominent S1 nuclease-sensitive and bromoacetaldehyde-modified sites were located within the TATA boxes of each 98-base-pair tandem repeat. Further studies revealed that the S1 nuclease-sensitive site in the first TATA box (proximal to the origin) was approximately 50-fold stronger than the site in the second TATA box (distal from the origin). Deletion of the first TATA box drastically reduced the extent of bromoacetaldehyde modification in the second TATA box, whereas deletion of the second TATA box had little or no effect on the reactivity at the first TATA box. Hence, the biological and conformational role of the second TATA box remains unclear. No supercoil-induced relaxation was found, and reactions with the probes were not pH dependent. Also, fragments containing this regulatory region did not appear to be bent, although the A+T-rich segment contained a tract of eight consecutive A's. We conclude that the regulatory region of JCV contains non-B, but right-handed, DNA conformations which account for this behavior.

Citing Articles

Host-Immune Interactions in JC Virus Reactivation and Development of Progressive Multifocal Leukoencephalopathy (PML).

Khalili A, Craigie M, Donadoni M, Sariyer I J Neuroimmune Pharmacol. 2019; 14(4):649-660.

PMID: 31452013 PMC: 6898772. DOI: 10.1007/s11481-019-09877-8.

Sp1 sites in the noncoding control region of BK polyomavirus are key regulators of bidirectional viral early and late gene expression.

Bethge T, Hachemi H, Manzetti J, Gosert R, Schaffner W, Hirsch H J Virol. 2015; 89(6):3396-411.

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Molecular biology, epidemiology, and pathogenesis of progressive multifocal leukoencephalopathy, the JC virus-induced demyelinating disease of the human brain.

Ferenczy M, Marshall L, Nelson C, Atwood W, Nath A, Khalili K Clin Microbiol Rev. 2012; 25(3):471-506.

PMID: 22763635 PMC: 3416490. DOI: 10.1128/CMR.05031-11.

Cooperative action of cellular proteins YB-1 and Pur alpha with the tumor antigen of the human JC polyomavirus determines their interaction with the viral lytic control element.

Chen N, Chang C, Gallia G, Kerr D, Johnson E, Krachmarov C Proc Natl Acad Sci U S A. 1995; 92(4):1087-91.

PMID: 7862639 PMC: 42642. DOI: 10.1073/pnas.92.4.1087.

Identification of critical elements within the JC virus DNA replication origin.

Lynch K, Frisque R J Virol. 1990; 64(12):5812-22.

PMID: 2173768 PMC: 248737. DOI: 10.1128/JVI.64.12.5812-5822.1990.

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