Regulation of Phosphatidylinositol Kinase Activity in Saccharomyces Cerevisiae

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1988 Feb 1

PMID 2828327

Citations 1

Authors

K M Holland

M J Homann

C J Belunis

G M Carman

Affiliations

Soon will be listed here.

Abstract

The effects of growth phase and carbon source on membrane-associated phosphatidylinositol kinase in cell extracts of Saccharomyces cerevisiae were examined. Phosphatidylinositol kinase activity increased 2- and 2.5-fold in glucose- and glycerol-grown cells, respectively, in the stationary phase as compared with the exponential phase of growth. The increase in phosphatidylinositol kinase activity in the stationary phase of growth correlated with an increase in the relative amounts of phosphatidylinositol 4-phosphate, the product of the reaction. The increase in phosphatidylinositol kinase activity was not due to the presence of water-soluble effector molecules in cell extracts as indicated by mixing experiments. Phosphatidylinositol kinase activity decreased in cell extracts of exponential-phase cells preincubated under phosphorylation conditions which favor cyclic AMP-dependent protein kinase activity. Phosphatidylinositol kinase activity was not affected in cell extracts of stationary-phase cells preincubated under phosphorylation conditions.

Citing Articles

Regulation of phosphatidylglycerolphosphate synthase in Saccharomyces cerevisiae by factors affecting mitochondrial development.

Gaynor P, Hubbell S, Schmidt A, Lina R, Minskoff S, Greenberg M J Bacteriol. 1991; 173(19):6124-31.

PMID: 1655699 PMC: 208360. DOI: 10.1128/jb.173.19.6124-6131.1991.

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