Functional Conservation and Coherence of HIV-1 Subtype A Vpu Alleles

Overview

Journal Sci Rep

Specialty Science

Date 2017 Mar 10

PMID 28273896

Citations 3

Authors

Bizhan Romani

Amirarsalan Kavyanifard

Elham Allahbakhshi

Affiliations

Soon will be listed here.

Abstract

Functional studies of HIV-1 proteins are normally conducted using lab adapted strains of HIV-1. The extent of those functions in clinical strains is sometimes unknown. In this study, we amplified and sequenced HIV-1 Vpu from 10 Iranian patients infected with HIV-1. Phylogenetic analysis indicated that the Vpu alleles were closely related to the CRF35_AD from Iran and subtype A Vpu. We addressed some of the well-established functions of the HIV-1 Vpu, as well as some of its recently reported functions. Ability of the clinical strains of subtype A Vpu alleles for downregulation of CD4 was similar to that of the lab adapted NL4.3 Vpu. Majority of the subtype A Vpu alleles performed stronger than NL4.3 Vpu for downregulation of SNAT1. The Vpu alleles differentially induced downregulation of HLA-C, ranging from no effect to 88% downregulation of surface HLA-C. Downregulation of tetherin and enhancement of virus release was similar for the subtype A Vpu alleles and NL4.3. Subtype A Vpu alleles were more potent when compared with NL4.3 for inhibition of NF-κB activation. Our study shows that subtype A Vpu alleles exert the classical functions of HIV-1 Vpu.

Citing Articles

Convergent Evolution of HLA-C Downmodulation in HIV-1 and HIV-2.

Hopfensperger K, Richard J, Sturzel C, Bibollet-Ruche F, Apps R, Leoz M mBio. 2020; 11(4).

PMID: 32665270 PMC: 7360927. DOI: 10.1128/mBio.00782-20.

HLA Class I Downregulation by HIV-1 Variants from Subtype C Transmission Pairs.

Ende Z, Deymier M, Claiborne D, Prince J, Monaco D, Kilembe W J Virol. 2018; 92(7).

PMID: 29321314 PMC: 5972908. DOI: 10.1128/JVI.01633-17.

Erratum: Functional conservation and coherence of HIV-1 subtype A Vpu alleles.

Romani B, Kavyanifard A, Allahbakhshi E Sci Rep. 2017; 7:46882.

PMID: 28849782 PMC: 5574054. DOI: 10.1038/srep46882.

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