In Situ Cellular Level Raman Spectroscopy of the Thyroid

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2017 Mar 9

PMID 28270975

Citations 4

Authors

Alan Wing Lun Law

Rafay Ahmed

Tsz Wing Cheung

Chun Yu Mak

Condon Lau

Affiliations

Soon will be listed here.

Abstract

We report a novel Raman spectroscopy method for in situ cellular level analysis of the thyroid. Thyroids are harvested from control and lithium treated mice. Lithium is used to treat bipolar disorder, but affects thyroid function. Raman spectra are acquired with a confocal setup (514 nm laser, 20 µm spot) focused on a follicular lumen. Raman peaks are observed at 1440, 1656, and 1746 cm, corresponding to tyrosine, an important amino acid for protein synthesis. Peaks are also observed at 563, 1087, 1265 and 1301 cm. With lithium, the tyrosine peaks increase, indicating tyrosine buildup. Raman spectroscopy can study the impact of many exogenous treatments on thyroid biochemistry.

Citing Articles

Raman Spectroscopy as a Potential Adjunct of Thyroid Nodule Evaluation: A Systematic Review.

Kujdowicz M, Janus D, Taczanowska-Niemczuk A, Lankosz M, Adamek D Int J Mol Sci. 2023; 24(20).

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Single red blood cell analysis reveals elevated hemoglobin in poikilocytes.

Tsui S, Ahmed R, Amjad N, Ahmed I, Yang J, Manno F J Biomed Opt. 2020; 25(1):1-13.

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Rapid and in situ optical detection of trace lithium in tissues.

Ahmed I, Yang J, Lun Law A, Manno F, Ahmed R, Zhang Y Biomed Opt Express. 2019; 9(9):4459-4471.

PMID: 30615723 PMC: 6157780. DOI: 10.1364/BOE.9.004459.

Elemental analysis of the thyroid by laser induced breakdown spectroscopy.

Ahmed I, Ahmed R, Yang J, Lun Law A, Zhang Y, Lau C Biomed Opt Express. 2017; 8(11):4865-4871.

PMID: 29188087 PMC: 5695937. DOI: 10.1364/BOE.8.004865.

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