Phosphoproteins in Extracellular Vesicles As Candidate Markers for Breast Cancer

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2017 Mar 9

PMID 28270605

Citations 179

Authors

I-Hsuan Chen

Liang Xue

Chuan-Chih Hsu

Juan Sebastian Paez Paez

Li Pan

Hillary Andaluz

Michael K Wendt

Anton B Iliuk

Jian-Kang Zhu

W Andy Tao

Affiliations

Soon will be listed here.

Abstract

The state of protein phosphorylation can be a key determinant of cellular physiology such as early-stage cancer, but the development of phosphoproteins in biofluids for disease diagnosis remains elusive. Here we demonstrate a strategy to isolate and identify phosphoproteins in extracellular vesicles (EVs) from human plasma as potential markers to differentiate disease from healthy states. We identified close to 10,000 unique phosphopeptides in EVs isolated from small volumes of plasma samples. Using label-free quantitative phosphoproteomics, we identified 144 phosphoproteins in plasma EVs that are significantly higher in patients diagnosed with breast cancer compared with healthy controls. Several biomarkers were validated in individual patients using paralleled reaction monitoring for targeted quantitation. This study demonstrates that the development of phosphoproteins in plasma EV as disease biomarkers is highly feasible and may transform cancer screening and monitoring.

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