» Articles » PMID: 28267624

A TaqMan-based QRT-PCR Assay for Senecavirus A Detection in Tissue Samples of Neonatal Piglets

Overview
Journal Mol Cell Probes
Date 2017 Mar 8
PMID 28267624
Citations 16
Authors
Affiliations
Soon will be listed here.
Abstract

This study describes a sensitive (1.3 × 10 genomic copies/μL) and specific TaqMan-based qRT-PCR assay able to detect and quantify SVA RNA in porcine biological samples. The technique represents an efficient tool for the virus diagnosis and assessment of SVA load in tissues of infected animals and for epidemiological studies.

Citing Articles

Interfering factors in the diagnosis of Senecavirus A.

Fonseca Junior A, Laguardia-Nascimento M, Barbosa A, da Silva Goncalves V, Camargos M Mol Biol Rep. 2024; 51(1):777.

PMID: 38904698 DOI: 10.1007/s11033-024-09692-2.


Development of a CRISPR/Cas12a-based fluorescent detection method of Senecavirus A.

He W, Liao K, Li R, Peng W, Qian B, Zeng D BMC Vet Res. 2024; 20(1):258.

PMID: 38877537 PMC: 11179212. DOI: 10.1186/s12917-024-04116-6.


Inactivation of highly transmissible livestock and avian viruses including influenza A and Newcastle disease virus for molecular diagnostics.

Welch J, Shrestha R, Hutchings H, Pal N, Levings R, Robbe-Austerman S Front Vet Sci. 2024; 11:1304022.

PMID: 38515532 PMC: 10955088. DOI: 10.3389/fvets.2024.1304022.


Construction and characterization of a full-length infectious clone of an emerging senecavirus A strain.

Wang H, Mo Y, Liu W, Niu C, He Q, Ren T Arch Virol. 2024; 169(2):25.

PMID: 38214826 DOI: 10.1007/s00705-023-05951-y.


Advances in the differential molecular diagnosis of vesicular disease pathogens in swine.

Chen W, Wang W, Wang X, Li Z, Wu K, Li X Front Microbiol. 2022; 13:1019876.

PMID: 36386633 PMC: 9641196. DOI: 10.3389/fmicb.2022.1019876.