Efficient and Rapid Generation of Large Genomic Variants in Rats and Mice Using CRISMERE

Overview

Journal Sci Rep

Specialty Science

Date 2017 Mar 8

PMID 28266534

Citations 43

Authors

Marie-Christine Birling

Laurence Schaeffer

Philippe Andre

Loic Lindner

Damien Marechal

Abdel Ayadi

Tania Sorg

Guillaume Pavlovic

Yann Herault

Affiliations

Soon will be listed here.

Abstract

Modelling Down syndrome (DS) in mouse has been crucial for the understanding of the disease and the evaluation of therapeutic targets. Nevertheless, the modelling so far has been limited to the mouse and, even in this model, generating duplication of genomic regions has been labour intensive and time consuming. We developed the CRISpr MEdiated REarrangement (CRISMERE) strategy, which takes advantage of the CRISPR/Cas9 system, to generate most of the desired rearrangements from a single experiment at much lower expenses and in less than 9 months. Deletions, duplications, and inversions of genomic regions as large as 24.4 Mb in rat and mouse founders were observed and germ line transmission was confirmed for fragment as large as 3.6 Mb. Interestingly we have been able to recover duplicated regions from founders in which we only detected deletions. CRISMERE is even more powerful than anticipated it allows the scientific community to manipulate the rodent and probably other genomes in a fast and efficient manner which was not possible before.

Citing Articles

Genome engineering with Cas9 and AAV repair templates, successes and pitfalls.

Birling M, Herault Y, Pavlovic G Mamm Genome. 2025; .

PMID: 39804466 DOI: 10.1007/s00335-024-10099-4.

Long-read sequencing for fast and robust identification of correct genome-edited alleles: PCR-based and Cas9 capture methods.

McCabe C, Price P, Codner G, Allan A, Caulder A, Christou S PLoS Genet. 2024; 20(3):e1011187.

PMID: 38457464 PMC: 10954187. DOI: 10.1371/journal.pgen.1011187.

Genotyping Protocols for Genetically Engineered Mice.

Limaye A, Cho K, Hall B, Khillan J, Kulkarni A Curr Protoc. 2023; 3(11):e929.

PMID: 37984376 PMC: 10754054. DOI: 10.1002/cpz1.929.

CRISMERE Chromosome Engineering in Mouse and Rat.

Schaeffer L, Lindner L, Pavlovic G, Herault Y, Birling M Methods Mol Biol. 2023; 2631:277-297.

PMID: 36995673 DOI: 10.1007/978-1-0716-2990-1_12.

Gene Editing in Mouse Zygotes Using the CRISPR/Cas9 System.

Wefers B, Wurst W, Kuhn R Methods Mol Biol. 2023; 2631:207-230.

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References

Mullins L, Mullins J . Insights from the rat genome sequence. Genome Biol. 2004; 5(5):221. PMC: 416459. DOI: 10.1186/gb-2004-5-5-221. View

Arque G, Fotaki V, Fernandez D, Martinez de Lagran M, Arbones M, Dierssen M . Impaired spatial learning strategies and novel object recognition in mice haploinsufficient for the dual specificity tyrosine-regulated kinase-1A (Dyrk1A). PLoS One. 2008; 3(7):e2575. PMC: 2481280. DOI: 10.1371/journal.pone.0002575. View

Wang L, Shao Y, Guan Y, Li L, Wu L, Chen F . Large genomic fragment deletion and functional gene cassette knock-in via Cas9 protein mediated genome editing in one-cell rodent embryos. Sci Rep. 2015; 5:17517. PMC: 4664917. DOI: 10.1038/srep17517. View

Jiang X, Liu C, Yu T, Zhang L, Meng K, Xing Z . Genetic dissection of the Down syndrome critical region. Hum Mol Genet. 2015; 24(22):6540-51. PMC: 4614710. DOI: 10.1093/hmg/ddv364. View

Altafaj X, Dierssen M, Baamonde C, Marti E, Visa J, Guimera J . Neurodevelopmental delay, motor abnormalities and cognitive deficits in transgenic mice overexpressing Dyrk1A (minibrain), a murine model of Down's syndrome. Hum Mol Genet. 2001; 10(18):1915-23. DOI: 10.1093/hmg/10.18.1915. View

Bronicki L, Redin C, Drunat S, Piton A, Lyons M, Passemard S . Ten new cases further delineate the syndromic intellectual disability phenotype caused by mutations in DYRK1A. Eur J Hum Genet. 2015; 23(11):1482-7. PMC: 4613470. DOI: 10.1038/ejhg.2015.29. View

Watanabe M, Osada J, Aratani Y, Kluckman K, Reddick R, MALINOW M . Mice deficient in cystathionine beta-synthase: animal models for mild and severe homocyst(e)inemia. Proc Natl Acad Sci U S A. 1995; 92(5):1585-9. PMC: 42564. DOI: 10.1073/pnas.92.5.1585. View

Dierssen M, Herault Y, Estivill X . Aneuploidy: from a physiological mechanism of variance to Down syndrome. Physiol Rev. 2009; 89(3):887-920. DOI: 10.1152/physrev.00032.2007. View

Kraft K, Geuer S, Will A, Chan W, Paliou C, Borschiwer M . Deletions, Inversions, Duplications: Engineering of Structural Variants using CRISPR/Cas in Mice. Cell Rep. 2015; 10(5):833-839. DOI: 10.1016/j.celrep.2015.01.016. View

10.

Lee H, Kweon J, Kim E, Kim S, Kim J . Targeted chromosomal duplications and inversions in the human genome using zinc finger nucleases. Genome Res. 2011; 22(3):539-48. PMC: 3290789. DOI: 10.1101/gr.129635.111. View

11.

Adams D, Biggs P, Cox T, Davies R, van der Weyden L, Jonkers J . Mutagenic insertion and chromosome engineering resource (MICER). Nat Genet. 2004; 36(8):867-71. DOI: 10.1038/ng1388. View

12.

Nigam R, Laulederkind S, Hayman G, Smith J, Wang S, Lowry T . Rat Genome Database: a unique resource for rat, human, and mouse quantitative trait locus data. Physiol Genomics. 2013; 45(18):809-16. PMC: 3783816. DOI: 10.1152/physiolgenomics.00065.2013. View

13.

Brault V, Pereira P, Duchon A, Herault Y . Modeling chromosomes in mouse to explore the function of genes, genomic disorders, and chromosomal organization. PLoS Genet. 2006; 2(7):e86. PMC: 1500809. DOI: 10.1371/journal.pgen.0020086. View

14.

van Bon B, Coe B, Bernier R, Green C, Gerdts J, Witherspoon K . Disruptive de novo mutations of DYRK1A lead to a syndromic form of autism and ID. Mol Psychiatry. 2015; 21(1):126-32. PMC: 4547916. DOI: 10.1038/mp.2015.5. View

15.

Ruf S, Symmons O, Uslu V, Dolle D, Hot C, Ettwiller L . Large-scale analysis of the regulatory architecture of the mouse genome with a transposon-associated sensor. Nat Genet. 2011; 43(4):379-86. DOI: 10.1038/ng.790. View

16.

Li J, Shou J, Guo Y, Tang Y, Wu Y, Jia Z . Efficient inversions and duplications of mammalian regulatory DNA elements and gene clusters by CRISPR/Cas9. J Mol Cell Biol. 2015; 7(4):284-98. PMC: 4524425. DOI: 10.1093/jmcb/mjv016. View

17.

Guedj F, Lopes Pereira P, Najas S, Barallobre M, Chabert C, Souchet B . DYRK1A: a master regulatory protein controlling brain growth. Neurobiol Dis. 2012; 46(1):190-203. DOI: 10.1016/j.nbd.2012.01.007. View

18.

Duchon A, Besson V, Lopes Pereira P, Magnol L, Herault Y . Inducing segmental aneuploid mosaicism in the mouse through targeted asymmetric sister chromatid event of recombination. Genetics. 2008; 180(1):51-9. PMC: 2535701. DOI: 10.1534/genetics.108.092312. View

19.

Pan D, Zhang L . Tandemly arrayed genes in vertebrate genomes. Comp Funct Genomics. 2008; :545269. PMC: 2547482. DOI: 10.1155/2008/545269. View

20.

Growney J, Shigematsu H, Li Z, Lee B, Adelsperger J, Rowan R . Loss of Runx1 perturbs adult hematopoiesis and is associated with a myeloproliferative phenotype. Blood. 2005; 106(2):494-504. PMC: 1895175. DOI: 10.1182/blood-2004-08-3280. View