Association of Heat Stress Protein 90 and 70 Gene Polymorphism with Adaptability Traits in Indian Sheep (Ovis Aries)

Overview

Journal Cell Stress Chaperones

Publisher Elsevier

Specialty Cell Biology

Date 2017 Mar 8

PMID 28265807

Citations 10

Authors

K M Singh

S Singh

I Ganguly

Raja K Nachiappan

A Ganguly

R Venkataramanan

A Chopra

H K Narula

Affiliations

Soon will be listed here.

Abstract

Heat stress proteins assist cellular proteins in the acquisition of native structure. The present research was conducted to study how thermo-tolerance is modulated by HSP90 and HSP70 gene polymorphism and its association with hemato-physio-biochemical parameters, supported by their expression profiles in Chokla, Magra, Marwari, and Madras Red sheep breeds. Least square analysis revealed significant effect (P < 0.05) of season and breed on all the physiological parameters, i.e., temperature, respiratory rate, and pulse rate (a.m. and p.m.), as well as hematological parameters like Hb, packed cell volume, total erythrocyte count (TEC), neutrophil/lymphocyte (N/L) ratio, and total leukocyte count (TLC). There was a significant influence (P < 0.05) of breed on biochemical parameters such as glucose, SGOT, phosphorous, triglyceride, and cholesterol. Eight fragments were amplified and sequenced in HSP90, and 70 genes and 13 single-nucleotide polymorphisms (SNPs) were identified. Tetra-primer amplification refractory mutation system PCR, PCR-RFLP, and allele-specific PCR genotyping protocols were developed for large-scale genotyping of five SNPs. A significant difference (P < 0.05) of rectal temperature (a.m.), respiratory rate (p.m.), triglyceride, and total protein was observed at SNP01; albumin at SNP2; pulse rate (p.m.) at SNP3; and rectal temperature (p.m.), pulse rate (p.m.), Hb (g/dL), and N/L ratio at SNP4 and TLC at SNP5. Gene expression analysis revealed higher expression in less adapted animals with Madras Red < Magra < Chokla < Marwari expression pattern [corrected]. Predominant allele was found to be superior in most of the SNPs (SNP1-4) indicating the selection acting in directional manner (positive selection). Finally, it is concluded that TACCA haplotype combination of SNP1-SNP2-SNP3-SNP4-SNP5 might be of some selection advantage for the identification of animals more adaptable to heat stress.

Citing Articles

Applications of Next-Generation Sequencing Technologies and Statistical Tools in Identifying Pathways and Biomarkers for Heat Tolerance in Livestock.

Kalaignazhal G, Sejian V, Velayudhan S, Mishra C, Rebez E, Chauhan S Vet Sci. 2024; 11(12).

PMID: 39728955 PMC: 11680151. DOI: 10.3390/vetsci11120616.

The Effects of Housing on Growth, Immune Function and Antioxidant Status of Young Female Lambs in Cold Conditions.

Xiao J, Guo W, Han Z, Xu Y, Xing Y, Phillips C Animals (Basel). 2024; 14(3).

PMID: 38338161 PMC: 10854601. DOI: 10.3390/ani14030518.

Feed efficiency in dairy sheep: An insight from the milk transcriptome.

Suarez-Vega A, Frutos P, Gutierrez-Gil B, Esteban-Blanco C, Toral P, Arranz J Front Vet Sci. 2023; 10:1122953.

PMID: 37077950 PMC: 10106586. DOI: 10.3389/fvets.2023.1122953.

Analysis of Potential Genes and Economic Parameters Associated with Growth and Heat Tolerance in Sheep ().

Ibrahim S, Al-Sharif M, Younis F, Ateya A, Abdo M, Fericean L Animals (Basel). 2023; 13(3).

PMID: 36766241 PMC: 9913162. DOI: 10.3390/ani13030353.

Validation of SNP markers for thermotolerance adaptation in Ovis aries adapted to different climatic regions using KASP-PCR technique.

Astuti P, Ilie D, Gavojdian D, Wanjala G, Badaoui B, Ohran H Sci Rep. 2022; 12(1):22348.

PMID: 36572697 PMC: 9792578. DOI: 10.1038/s41598-022-26909-1.

References

Marcos-Carcavilla A, Moreno C, Serrano M, Laurent P, Cribiu E, Andreoletti O . Polymorphisms in the HSP90AA1 5' flanking region are associated with scrapie incubation period in sheep. Cell Stress Chaperones. 2009; 15(4):343-9. PMC: 3082647. DOI: 10.1007/s12192-009-0149-2. View

Charoensook R, Gatphayak K, Sharifi A, Chaisongkram C, Brenig B, Knorr C . Polymorphisms in the bovine HSP90AB1 gene are associated with heat tolerance in Thai indigenous cattle. Trop Anim Health Prod. 2011; 44(4):921-8. PMC: 3289787. DOI: 10.1007/s11250-011-9989-8. View

Kumar A, Ashraf S, Goud T, Grewal A, Singh S, Yadav B . Expression profiling of major heat shock protein genes during different seasons in cattle (Bos indicus) and buffalo (Bubalus bubalis) under tropical climatic condition. J Therm Biol. 2015; 51:55-64. DOI: 10.1016/j.jtherbio.2015.03.006. View

Dangi S, Gupta M, Maurya D, Yadav V, Panda R, Singh G . Expression profile of HSP genes during different seasons in goats (Capra hircus). Trop Anim Health Prod. 2012; 44(8):1905-12. DOI: 10.1007/s11250-012-0155-8. View

Lei L, Yu J, Bao E . Expression of heat shock protein 90 (Hsp90) and transcription of its corresponding mRNA in broilers exposed to high temperature. Br Poult Sci. 2009; 50(4):504-11. DOI: 10.1080/00071660903110851. View

Park H, Ahn I, Lee H . Expression of heat shock protein 70 in the thermally stressed antarctic clam Laternula elliptica. Cell Stress Chaperones. 2007; 12(3):275-82. PMC: 1971231. DOI: 10.1379/csc-271.1. View

Rimoldi S, Lasagna E, Sarti F, Marelli S, Cozzi M, Bernardini G . Expression profile of six stress-related genes and productive performances of fast and slow growing broiler strains reared under heat stress conditions. Meta Gene. 2015; 6:17-25. PMC: 4556841. DOI: 10.1016/j.mgene.2015.08.003. View

Felver-Gant J, Mack L, Dennis R, Eicher S, Cheng H . Genetic variations alter physiological responses following heat stress in 2 strains of laying hens. Poult Sci. 2012; 91(7):1542-51. DOI: 10.3382/ps.2011-01988. View

Al-Murrani W, Kassab A . Heterophil/lymphocyte ratio as a selection criterion for heat resistance in domestic fowls. Br Poult Sci. 1997; 38(2):159-63. DOI: 10.1080/00071669708417962. View

10.

Cleries R, Galvez J, Espino M, Ribes J, Nunes V, de Heredia M . BootstRatio: A web-based statistical analysis of fold-change in qPCR and RT-qPCR data using resampling methods. Comput Biol Med. 2012; 42(4):438-45. DOI: 10.1016/j.compbiomed.2011.12.012. View

11.

Pfaffl M . A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001; 29(9):e45. PMC: 55695. DOI: 10.1093/nar/29.9.e45. View

12.

Marcos-Carcavilla A, Mutikainen M, Gonzalez C, Calvo J, Kantanen J, Sanz A . A SNP in the HSP90AA1 gene 5' flanking region is associated with the adaptation to differential thermal conditions in the ovine species. Cell Stress Chaperones. 2009; 15(1):67-81. PMC: 2866970. DOI: 10.1007/s12192-009-0123-z. View

13.

SCHLESINGER M . Heat shock proteins. J Biol Chem. 1990; 265(21):12111-4. View

14.

Boston R, Viitanen P, Vierling E . Molecular chaperones and protein folding in plants. Plant Mol Biol. 1996; 32(1-2):191-222. DOI: 10.1007/BF00039383. View

15.

Salces-Ortiz J, Gonzalez C, Martinez M, Mayoral T, Calvo J, Serrano M . Looking for adaptive footprints in the HSP90AA1 ovine gene. BMC Evol Biol. 2015; 15:7. PMC: 4351680. DOI: 10.1186/s12862-015-0280-x. View

16.

Banerjee D, Upadhyay R, Chaudhary U, Kumar R, Singh S, Ashutosh . Seasonal variation in expression pattern of genes under HSP70 : Seasonal variation in expression pattern of genes under HSP70 family in heat- and cold-adapted goats (Capra hircus). Cell Stress Chaperones. 2013; 19(3):401-8. PMC: 3982034. DOI: 10.1007/s12192-013-0469-0. View

17.

Salces-Ortiz J, Gonzalez C, Moreno-Sanchez N, Calvo J, Perez-Guzman M, Serrano M . Ovine HSP90AA1 expression rate is affected by several SNPs at the promoter under both basal and heat stress conditions. PLoS One. 2013; 8(6):e66641. PMC: 3691178. DOI: 10.1371/journal.pone.0066641. View