Structure and Transcription Analysis of the Gene Encoding a Cellobiase from Agrobacterium Sp. Strain ATCC 21400

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1988 Jan 1

PMID 2826395

Citations 16

Authors

W W Wakarchuk

N M Greenberg

D G Kilburn

R C Miller Jr

R A Warren

Affiliations

Soon will be listed here.

Abstract

The DNA sequence was determined for the cloned Agrobacterium sp. strain ATCC 21400 beta-glucosidase gene, abg. High-resolution nuclease S1 protection studies were used to map the abg mRNA 5' and 3' termini. A putative abg promoter was identified whose sequence shows similarities to the consensus promoter of Escherichia coli and with the nif promoter regions of Klebsiella. The abg coding sequence was 1,374 nucleotides long. The molecular weight of the enzyme, based on the predicted amino acid sequence, was 51,000. The observed Mr was 50,000 to 52,000. A region of deduced protein sequence was homologous to a region from two other beta-glucosidase sequences. This region of homology contained a putative active site by analogy with the active site of hen egg white lysozyme.

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References

Day A, Withers S . The purification and characterization of a beta-glucosidase from Alcaligenes faecalis. Biochem Cell Biol. 1986; 64(9):914-22. DOI: 10.1139/o86-122. View

Rosenberg M, Court D . Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979; 13:319-53. DOI: 10.1146/annurev.ge.13.120179.001535. View

Stoppok W, Rapp P, Wagner F . Formation, Location, and Regulation of Endo-1,4-beta-Glucanases and beta-Glucosidases from Cellulomonas uda. Appl Environ Microbiol. 1982; 44(1):44-53. PMC: 241966. DOI: 10.1128/aem.44.1.44-53.1982. View

Grosjean H, Fiers W . Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes. Gene. 1982; 18(3):199-209. DOI: 10.1016/0378-1119(82)90157-3. View

Zoller M, Smith M . Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors. Methods Enzymol. 1983; 100:468-500. DOI: 10.1016/0076-6879(83)00074-9. View

Gold L, Pribnow D, Schneider T, Shinedling S, Singer B, Stormo G . Translational initiation in prokaryotes. Annu Rev Microbiol. 1981; 35:365-403. DOI: 10.1146/annurev.mi.35.100181.002053. View

Han Y, Srinivasan V . Purification and characterization of beta-glucosidase of Alcaligenes faecalis. J Bacteriol. 1969; 100(3):1355-63. PMC: 250337. DOI: 10.1128/jb.100.3.1355-1363.1969. View

Zappe H, Jones D, Woods D . Cloning and expression of Clostridium acetobutylicum endoglucanase, cellobiase and amino acid biosynthesis genes in Escherichia coli. J Gen Microbiol. 1986; 132(5):1367-72. DOI: 10.1099/00221287-132-5-1367. View

LEGLER G, Harder A . Amino acid sequence at the active site of beta-glucosidase A from bitter almonds. Biochim Biophys Acta. 1978; 524(1):102-8. DOI: 10.1016/0005-2744(78)90108-0. View

10.

Messing J . New M13 vectors for cloning. Methods Enzymol. 1983; 101:20-78. DOI: 10.1016/0076-6879(83)01005-8. View

11.

Kim Y, Ahn K, Beppu T, Uozumi T . Nucleotide sequence of the nifLA operon of Klebsiella oxytoca NG13 and characterization of the gene products. Mol Gen Genet. 1986; 205(2):253-9. DOI: 10.1007/BF00430436. View

12.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

13.

HAWLEY D, McClure W . Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 1983; 11(8):2237-55. PMC: 325881. DOI: 10.1093/nar/11.8.2237. View

14.

Warren R, Beck C, Gilkes N, Kilburn D, Langsford M, Miller Jr R . Sequence conservation and region shuffling in an endoglucanase and an exoglucanase from Cellulomonas fimi. Proteins. 1986; 1(4):335-41. DOI: 10.1002/prot.340010407. View

15.

Vieira J, Messing J . Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985; 33(1):103-19. DOI: 10.1016/0378-1119(85)90120-9. View

16.

Greenberg N, Warren R, Kilburn D, Miller Jr R . Regulation, initiation, and termination of the cenA and cex transcripts of Cellulomonas fimi. J Bacteriol. 1987; 169(2):646-53. PMC: 211827. DOI: 10.1128/jb.169.2.646-653.1987. View

17.

Kohchi C, Toh-E A . Nucleotide sequence of Candida pelliculosa beta-glucosidase gene. Nucleic Acids Res. 1985; 13(17):6273-82. PMC: 321952. DOI: 10.1093/nar/13.17.6273. View

18.

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

19.

ONeill G, Goh S, Warren R, Kilburn D, Miller Jr R . Structure of the gene encoding the exoglucanase of Cellulomonas fimi. Gene. 1986; 44(2-3):325-30. DOI: 10.1016/0378-1119(86)90197-6. View

20.

Varenne S, Buc J, Lloubes R, Lazdunski C . Translation is a non-uniform process. Effect of tRNA availability on the rate of elongation of nascent polypeptide chains. J Mol Biol. 1984; 180(3):549-76. DOI: 10.1016/0022-2836(84)90027-5. View