Marker Rescue and Partial Replication of Bacteriophage T7 DNA

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1978 Dec 1

PMID 282630

Citations 11

Authors

K B Burck

R C Miller Jr

Affiliations

Soon will be listed here.

Abstract

Experiments reported here show that some UV-irradiated wild-type T7 phage markers can be rescued efficiently by coinfection with T7 amber mutant phage in a permissive host. Other results show that the segments of a UV-irradiated genome that replicate efficiently are those that also are rescued efficiently during a marker rescue experiment. At higher doses, fewer markers are rescued efficiently and fewer segments of the genome replicate efficiently. The results clearly indicate that the probability of marker rescue is correlated with the ability of the DNA containing the marker to replicate. Sucrose density gradient analysis shows that UV irradiation does not produce double-strand scissions in T7 DNA at doses used here. Therefore, the partial replication and rescue of markers from the left end of the genome is not due simply to injection of only the left end of the T7 DNA.

Citing Articles

Substitutions in the Escherichia coli RNA polymerase inhibitor T7 Gp2 that allow inhibition of transcription when the primary interaction interface between Gp2 and RNA polymerase becomes compromised.

Shadrin A, Sheppard C, Severinov K, Matthews S, Wigneshweraraj S Microbiology (Reading). 2012; 158(Pt 11):2753-2764.

PMID: 22977089 PMC: 3541766. DOI: 10.1099/mic.0.062547-0.

T7 phage protein Gp2 inhibits the Escherichia coli RNA polymerase by antagonizing stable DNA strand separation near the transcription start site.

Camara B, Liu M, Reynolds J, Shadrin A, Liu B, Kwok K Proc Natl Acad Sci U S A. 2010; 107(5):2247-52.

PMID: 20133868 PMC: 2836649. DOI: 10.1073/pnas.0907908107.

Two types of recombination hotspots in bacteriophage T4: one requires DNA damage and a replication origin and the other does not.

Doan P, Belanger K, Kreuzer K Genetics. 2001; 157(3):1077-87.

PMID: 11238396 PMC: 1461569. DOI: 10.1093/genetics/157.3.1077.

Partial replicas of UV-irradiated bacteriophage T4 genomes and their role in multiplicity reactivation.

Rayssiguier C, Kozinski A, DOERMANN A J Virol. 1980; 35(2):451-65.

PMID: 7441816 PMC: 288831. DOI: 10.1128/JVI.35.2.451-465.1980.

Multiplicity reactivation of bacteriophage T7 inactivated by methyl methanesulfonate.

Karska-Wysocki B, Mamet-Bratley M J Virol. 1984; 52(3):1009-10.

PMID: 6387176 PMC: 254631. DOI: 10.1128/JVI.52.3.1009-1010.1984.

References

ELLISON S, FEINER R, Hill R . A host effect on bacteriophage survival after ultraviolet irradiation. Virology. 1960; 11:294-6. DOI: 10.1016/0042-6822(60)90069-6. View

Denhardt D . A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966; 23(5):641-6. DOI: 10.1016/0006-291x(66)90447-5. View

STEINBERG C, Edgar R . A critical test of a current theory of genetic recombination in bacteriophage. Genetics. 1962; 47:187-208. PMC: 1210322. DOI: 10.1093/genetics/47.2.187. View

BARRICELLI N, DOERMANN A . An analytical approach to the problems of phage recombination and reproduction. III. Cross reactivation. Virology. 1961; 13:460-76. DOI: 10.1016/0042-6822(61)90277-x. View

Kuemmerle N, Masker W . In vitro packaging of UV radiation-damaged DNA from bacteriophage T7. J Virol. 1977; 23(3):509-16. PMC: 515861. DOI: 10.1128/JVI.23.3.509-516.1977. View

Benbasat J, Burck K, Miller Jr R . Superinfection exclusion and lack of conservative transfer of bacteriophage T7 DNA. Virology. 1978; 87(1):164-71. DOI: 10.1016/0042-6822(78)90168-x. View

Langman L, Paetkau V, Scraba D, Miller Jr R, Roeder G, Sadowski P . The structure and maturation of intermediates in bacteriophage T7 DNA replication. Can J Biochem. 1978; 56(6):508-16. DOI: 10.1139/o78-078. View

Rayssiguier C, Vigier P . Genetic evidence for the existence of partial replicas of T4 genomes inactivated by irradiation under ultraviolet light.?*ULTRAVIOLET RAYS. Virology. 1977; 78(2):442-52. DOI: 10.1016/0042-6822(77)90121-0. View

McDonell M, Simon M, Studier F . Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977; 110(1):119-46. DOI: 10.1016/s0022-2836(77)80102-2. View

10.

DOERMANN A, Eiserling F, BOEHNER L . Genetic control of capsid length in bacteriophage T4. I. Isolation and preliminary description of four new mutants. J Virol. 1973; 12(2):374-85. PMC: 356633. DOI: 10.1128/JVI.12.2.374-385.1973. View

11.

Yudkin J . Sugar and disease. Nature. 1972; 239(5369):197-9. DOI: 10.1038/239197a0. View

12.

Schlegel R, Thomas Jr C . Some special structural features of intracellular bacteriophage T7 concatemers. J Mol Biol. 1972; 68(2):319-45. DOI: 10.1016/0022-2836(72)90216-1. View

13.

Studier F . Gene 0.3 of bacteriophage T7 acts to overcome the DNA restriction system of the host. J Mol Biol. 1975; 94(2):283-95. DOI: 10.1016/0022-2836(75)90083-2. View

14.

PAO C, SPEYER J . Order of injection of T7 bacteriophage DNA. J Virol. 1973; 11(6):1024-6. PMC: 355214. DOI: 10.1128/JVI.11.6.1024-1026.1973. View

15.

HOWE C, Buckley P, Carlson K, Kozinski A . Multiple and specific initiation of T4 DNA replication. J Virol. 1973; 12(1):130-48. PMC: 355239. DOI: 10.1128/JVI.12.1.130-148.1973. View

16.

Dressler D, Wolfson J, Magazin M . Initiation and reinitiation of DNA synthesis during replication of bacteriophage T7. Proc Natl Acad Sci U S A. 1972; 69(4):998-1002. PMC: 426612. DOI: 10.1073/pnas.69.4.998. View

17.

Studier F . Genetic analysis of non-essential bacteriophage T7 genes. J Mol Biol. 1973; 79(2):227-36. DOI: 10.1016/0022-2836(73)90002-8. View

18.

Rayssiguier C, VIGIER P . On the repair mechanism responsible for multiplicity reactivation in bacteriophage T4. Mol Gen Genet. 1972; 115(2):140-5. DOI: 10.1007/BF00277294. View

19.

Delius H, Howe C, Kozinski A . Structure of the replicating DNA from bacteriophage T4. Proc Natl Acad Sci U S A. 1971; 68(12):3049-53. PMC: 389588. DOI: 10.1073/pnas.68.12.3049. View

20.

Mosig G . A preferred origin and direction of bacteriophage T4 DNA replication. I. A gradient of allele frequencies in crosses between normal and small T4 particles. J Mol Biol. 1970; 53(3):503-14. DOI: 10.1016/0022-2836(70)90080-x. View