Plasmid and Bacteriophage Vectors for Excision of Intact Inserts

Overview

Journal Gene

Specialty Molecular Biology

Date 1987 Jan 1

PMID 2826295

Citations 50

Authors

R Lathe

J L Vilotte

A J Clark

Affiliations

Soon will be listed here.

Abstract

Plasmid (pPolyIII) and bacteriophage lambda (EMBL301) vectors are described in which sites for the rare-cutting enzymes SfiI and NotI (8-bp, recognition sequences) flank the polylinker cloning region. Intact DNA inserts for introduction into cultured cells or into the early embryo are readily excised from the vectors. General-purpose miniplasmid cloning vectors pPolyI and pPolyII are also described, and the utility of the bacteriophage lambda vector is demonstrated in the construction of a bovine genomic library.

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