Polyphyllin D, a Steroidal Saponin in Paris Polyphylla, Induces Apoptosis and Necroptosis Cell Death of Neuroblastoma Cells

Overview

Journal Pediatr Surg Int

Specialties General Surgery
Pediatrics

Date 2017 Mar 6

PMID 28260192

Citations 18

Authors

Shunsuke Watanabe

Tatuya Suzuki

Fujio Hara

Toshihiro Yasui

Naoko Uga

Atuki Naoe

Affiliations

Soon will be listed here.

Abstract

Purpose: Neuroblastoma is a refractory pediatric malignant solid tumor. The previous studies demonstrated that Polyphyllin D, the main constituent of Paris polyphylla, a traditional Chinese medicine, exerts an anti-tumor effect on many tumors. However, its effects against neuroblastomas are unclear.

Methods: We examined the anti-tumor effect of polyphyllin D in human neuroblastoma using IMR-32 and LA-N-2 cells, which exhibit MYCN gene amplification, and NB-69 cells, which do not exhibit MYCN gene amplification.

Results: All cell lines showed reduced cell viability in response to polyphyllin D treatment. No caspase-3/-7, -8, and -9 activity was observed in IMR-32 and LA-N-2 cells treated with polyphyllin D. In contrast, activation of caspase-3/-7, and -8 activity was observed in NB-69 cells. When polyphyllin D and specific inhibitors of RIPK3 involved in necroptosis were added to IMR-32 and LA-N-2 cell lines, polyphyllin D-induced cell death was inhibited.

Conclusion: Together, this indicates that the underlying mechanism of polyphyllin D-induced cell death in NB-69 cells is apoptosis, whereas the cell death of IMR-32 and LA-N-2 cells occurs by necroptosis. We continue research on this topic and look forward the discovery of a new therapeutic agent for neuroblastoma.

Citing Articles

Proteomics Analysis of Polyphyllin D-Treated Triple-Negative Breast Cancer Cells Reveal the Anticancer Mechanisms of Polyphyllin D.

Wei C, Mao A, Liu Y, Zhang Q, Pan G, Liu W Appl Biochem Biotechnol. 2023; 196(6):3148-3161.

PMID: 37624509 PMC: 11166742. DOI: 10.1007/s12010-023-04679-4.

Steroidal Saponins: Naturally Occurring Compounds as Inhibitors of the Hallmarks of Cancer.

Bouabdallah S, Al-Maktoum A, Amin A Cancers (Basel). 2023; 15(15).

PMID: 37568716 PMC: 10417465. DOI: 10.3390/cancers15153900.

Polyphyllin D punctures hypertrophic lysosomes to reverse drug resistance of hepatocellular carcinoma by targeting acid sphingomyelinase.

Wang Y, Chen Y, Gao G, Zheng Y, Yu N, Ouyang L Mol Ther. 2023; 31(7):2169-2187.

PMID: 37211762 PMC: 10362416. DOI: 10.1016/j.ymthe.2023.05.015.

Polyphyllin D induces necroptosis in neuroblastoma cells (IMR-32 and LA-N-2) in mice.

Watanabe S, Inoue M, Suzuki T, Kondo Y, Murayama M Pediatr Surg Int. 2023; 39(1):196.

PMID: 37160784 DOI: 10.1007/s00383-023-05425-x.

Necroptosis: A Pathogenic Negotiator in Human Diseases.

Chaouhan H, Vinod C, Mahapatra N, Yu S, Wang I, Chen K Int J Mol Sci. 2022; 23(21).

PMID: 36361505 PMC: 9655262. DOI: 10.3390/ijms232112714.

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