Amphiregulin Antibody and Reduction of Axial Elongation in Experimental Myopia

Overview

Journal EBioMedicine

Specialty Biomedical Engineering

Date 2017 Mar 4

PMID 28256400

Citations 15

Authors

Wen Jun Jiang

Hui Xin Song

Shao Yu Li

Bin Guo

Jian Feng Wu

Guo Ping Li

Da Dong Guo

De Long Shi

Hong Sheng Bi

Jost B Jonas

Affiliations

Soon will be listed here.

Abstract

To examine the mechanism of ocular axial elongation in myopia, guinea pigs (age: 2-3weeks) which either underwent unilateral or bilateral lens-induced myopization (group 1) or which were primarily myopic at baseline (group 2) received unilateral intraocular injections of amphiregulin antibody (doses: 5, 10, or 15μg) three times in intervals of 9days. A third group of emmetropic guinea pigs got intraocular unilateral injections of amphiregulin (doses: 0.25, 0.50 or 1.00ng, respectively). In each group, the contralateral eyes received intraocular injections of Ringer's solution. In intra-animal inter-eye comparison and intra-eye follow-up comparison in groups 1 and 2, the study eyes as compared to the contralateral eyes showed a dose-dependent reduction in axial elongation. In group 3, study eyes and control eyes did not differ significantly in axial elongation. Immunohistochemistry revealed amphiregulin labelling at the retinal pigment epithelium in eyes with lens-induced myopization and Ringer's solution injection, but not in eyes with amphiregulin antibody injection. Intraocular injections of amphiregulin-antibody led to a reduction of lens-induced axial myopic elongation and of the physiological eye enlargement in young guinea pigs. In contrast, intraocularly injected amphiregulin in a dose of ≤1ng did not show a significant effect. Amphiregulin may be one of several essential molecular factors for axial elongation.

Citing Articles

Intraocular Amphiregulin antibody and axial elongation in nonhuman primates.

Wang W, Nan Y, Huang T, Pu M, Jonas J Front Ophthalmol (Lausanne). 2024; 2:995157.

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Reduction of experimental ocular axial elongation by neuregulin-1 antibody.

Shi X, Dong L, Zhang R, Zhou W, Li Y, Wu H Front Med (Lausanne). 2023; 10:1277180.

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mTORC1 Signaling and Negative Lens-Induced Axial Elongation.

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Intravitreal injection of fibrillin 2 (Fbn2) recombinant protein for therapy of retinopathy in a retina-specific Fbn2 knock-down mouse model.

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Intravitreal Short-Hairpin RNA Attenuated Adeno-Associated Virus-Induced Knockdown of Amphiregulin and Axial Elongation in Experimental Myopia.

Dong L, Zhang R, Wu H, Li H, Zhou W, Shi X Invest Ophthalmol Vis Sci. 2023; 64(4):11.

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