Humoral Responses to Rv1733c, Rv0081, Rv1735c, and Rv1737c DosR Regulon-Encoded Proteins of in Individuals with Latent Tuberculosis Infection

Overview

Journal J Immunol Res

Publisher Wiley

Specialty Allergy & Immunology

Date 2017 Mar 4

PMID 28255560

Citations 10

Authors

Simon G Kimuda

Angela Nalwoga

Jonathan Levin

Kees L M C Franken

Tom H M Ottenhoff

Alison M Elliott

Stephen Cose

Irene Andia-Biraro

Affiliations

Soon will be listed here.

Abstract

Latent tuberculosis infection (LTBI) is evidence of immunological control of tuberculosis. Dormancy survival regulator (DosR) regulon-encoded proteins may have a role in the maintenance of LTBI. T cell responses to Rv1733c, Rv0081, Rv1735c, and Rv1737c DosR regulon-encoded proteins were found to be most frequent among household contacts of TB cases from Uganda compared to other DosR proteins, but antibody responses were not described. We characterized antibody responses to these proteins in individuals from Uganda. Antibodies to Rv1733c, Rv0081, Rv1735c, and Rv1737c DosR regulon-encoded proteins were measured in 68 uninfected individuals, 62 with LTBI, and 107 with active pulmonary tuberculosis (APTB) cases. There were no differences in the concentrations of antibodies to Rv0081, Rv1735c, and Rv1737c DosR regulon-encoded proteins between individuals with LTBI and APTB and those who were uninfected. LTBI was associated with higher concentrations of antibodies to Rv1733c in female participants [adjusted geometric mean ratio: 1.812, 95% confidence interval (CI): 1.105 2.973, and = 0.019] but not in males ( value for interaction = 0.060). Antibodies to the four DosR regulon-encoded proteins investigated may not serve as good biomarkers of LTBI in the general population. More of the proteome needs to be screened to identify proteins that induce strong antibody responses in LTBI.

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References

Phuah J, Mattila J, Lin P, Flynn J . Activated B cells in the granulomas of nonhuman primates infected with Mycobacterium tuberculosis. Am J Pathol. 2012; 181(2):508-14. PMC: 3409439. DOI: 10.1016/j.ajpath.2012.05.009. View

Davidow A, Kanaujia G, Shi L, Kaviar J, Guo X, Sung N . Antibody profiles characteristic of Mycobacterium tuberculosis infection state. Infect Immun. 2005; 73(10):6846-51. PMC: 1230945. DOI: 10.1128/IAI.73.10.6846-6851.2005. View

Oertelt-Prigione S . The influence of sex and gender on the immune response. Autoimmun Rev. 2011; 11(6-7):A479-85. DOI: 10.1016/j.autrev.2011.11.022. View

Mattos A, Chaves A, Franken K, Figueiredo B, Ferreira A, Ottenhoff T . Detection of IgG1 antibodies against Mycobacterium tuberculosis DosR and Rpf antigens in tuberculosis patients before and after chemotherapy. Tuberculosis (Edinb). 2016; 96:65-70. DOI: 10.1016/j.tube.2015.11.001. View

Ahmad S . Pathogenesis, immunology, and diagnosis of latent Mycobacterium tuberculosis infection. Clin Dev Immunol. 2011; 2011:814943. PMC: 3017943. DOI: 10.1155/2011/814943. View

Mack U, Migliori G, Sester M, Rieder H, Ehlers S, Goletti D . LTBI: latent tuberculosis infection or lasting immune responses to M. tuberculosis? A TBNET consensus statement. Eur Respir J. 2009; 33(5):956-73. DOI: 10.1183/09031936.00120908. View

Lu L, Chung A, Rosebrock T, Ghebremichael M, Yu W, Grace P . A Functional Role for Antibodies in Tuberculosis. Cell. 2016; 167(2):433-443.e14. PMC: 5526202. DOI: 10.1016/j.cell.2016.08.072. View

Betts J, Lukey P, Robb L, McAdam R, Duncan K . Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Mol Microbiol. 2002; 43(3):717-31. DOI: 10.1046/j.1365-2958.2002.02779.x. View

Gennaro M, Affouf M, Kanaujia G, Brusasca P, Mangura B, Reichman L . Antibody markers of incident tuberculosis among HIV-infected adults in the USA: a historical prospective study. Int J Tuberc Lung Dis. 2007; 11(6):624-31. View

10.

Sutherland J, Lalor M, Black G, Ambrose L, Loxton A, Chegou N . Analysis of host responses to Mycobacterium tuberculosis antigens in a multi-site study of subjects with different TB and HIV infection states in sub-Saharan Africa. PLoS One. 2013; 8(9):e74080. PMC: 3769366. DOI: 10.1371/journal.pone.0074080. View

11.

Eleftheriadis T, Tsiaga P, Antoniadi G, Liakopoulos V, Kortsaris A, Giannatos E . The value of serum antilipoarabinomannan antibody detection in the diagnosis of latent tuberculosis in hemodialysis patients. Am J Kidney Dis. 2005; 46(4):706-12. DOI: 10.1053/j.ajkd.2005.06.021. View

12.

Leyten E, Lin M, Franken K, Friggen A, Prins C, van Meijgaarden K . Human T-cell responses to 25 novel antigens encoded by genes of the dormancy regulon of Mycobacterium tuberculosis. Microbes Infect. 2006; 8(8):2052-60. DOI: 10.1016/j.micinf.2006.03.018. View

13.

Nielsen N, Soborg B, Borresen M, Andersson M, Koch A . Cytokine responses in relation to age, gender, body mass index, Mycobacterium tuberculosis infection, and otitis media among Inuit in Greenland. Am J Hum Biol. 2012; 25(1):20-8. DOI: 10.1002/ajhb.22332. View

14.

Schuck S, Mueller H, Kunitz F, Neher A, Hoffmann H, Franken K . Identification of T-cell antigens specific for latent mycobacterium tuberculosis infection. PLoS One. 2009; 4(5):e5590. PMC: 2680040. DOI: 10.1371/journal.pone.0005590. View

15.

Chen J, Wang S, Zhang Y, Su X, Wu J, Shao L . Rv1985c, a promising novel antigen for diagnosis of tuberculosis infection from BCG-vaccinated controls. BMC Infect Dis. 2010; 10:273. PMC: 2949761. DOI: 10.1186/1471-2334-10-273. View

16.

Black G, Thiel B, Ota M, Parida S, Adegbola R, Boom W . Immunogenicity of novel DosR regulon-encoded candidate antigens of Mycobacterium tuberculosis in three high-burden populations in Africa. Clin Vaccine Immunol. 2009; 16(8):1203-12. PMC: 2725533. DOI: 10.1128/CVI.00111-09. View

17.

Wood R, Liang H, Wu H, Middelkoop K, Oni T, Rangaka M . Changing prevalence of tuberculosis infection with increasing age in high-burden townships in South Africa. Int J Tuberc Lung Dis. 2010; 14(4):406-12. PMC: 2837545. View

18.

Diwan V, Thorson A . Sex, gender, and tuberculosis. Lancet. 1999; 353(9157):1000-1. DOI: 10.1016/S0140-6736(99)01318-5. View

19.

Siev M, Wilson D, Kainth S, Kasprowicz V, Feintuch C, Jenny-Avital E . Antibodies against Mycobacterial proteins as biomarkers for HIV-associated smear-negative tuberculosis. Clin Vaccine Immunol. 2014; 21(6):791-8. PMC: 4054248. DOI: 10.1128/CVI.00805-13. View

20.

Neyrolles O, Quintana-Murci L . Sexual inequality in tuberculosis. PLoS Med. 2009; 6(12):e1000199. PMC: 2788129. DOI: 10.1371/journal.pmed.1000199. View