Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients

Overview

Journal Mediators Inflamm

Publisher Wiley

Specialties Biochemistry
Pathology

Date 2017 Mar 4

PMID 28255201

Citations 10

Authors

David Heftrig

Ramona Sturm

Elsie Oppermann

Kerstin Kontradowitz

Katrin Jurida

Lukas Schimunek

Mathias Woschek

Ingo Marzi

Borna Relja

Affiliations

Soon will be listed here.

Abstract

. Trauma patients (TP) frequently develop an imbalanced immune response that often causes infectious postinjury complications. Monocytes show a diminished capability of both producing proinflammatory cytokines and antigen presentation after trauma. TLR2, TLR4, and TLR9 recognize pathogens and subsequently activate monocytes. While there are conflictive data about TLR2 and TLR4 expression after trauma, no studies about the expression of TLR2, TLR4, TLR9, and HLA-DR on monocytes from TP after their secondary ex vivo-in vitro "hit" have been reported. . Ex vivo-in vitro lipopolysaccharide- (LPS-) stimulated blood from TP showed diminished interleukin- (IL-) 1-release in TP for five postinjury days compared to healthy volunteers (HV). The recovery was observed at day 5. In parallel, monocytes from TP showed an impaired capability of TLR2, TLR4, and TLR9 expression after secondary stimulation compared to HV, while the measurement of unstimulated samples showed significant reduction of TLR4 and TLR9 at ED. Furthermore, HLA-DR decreased after trauma and was even more profound by stimulation of monocytes. Ratio of monocytes to leukocytes was significantly increased at days 6 and 7 after trauma compared to HV. . Impaired expression of TLRs and HLA-DR in acute inflammatory conditions may be responsible for the well-described monocyte paralysis after severe trauma.

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References

Hu C, Xu X, Liang H, Lu F, Shen L, Hao T . [Changes in human leukocyte antigen-DR expression on monocytes and its value of prediction on infection complication in trauma patients]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2004; 16(4):193-7. View

Pfeifer R, Tarkin I, Rocos B, Pape H . Patterns of mortality and causes of death in polytrauma patients--has anything changed?. Injury. 2009; 40(9):907-11. DOI: 10.1016/j.injury.2009.05.006. View

Gentile L, Cuenca A, Efron P, Ang D, Bihorac A, McKinley B . Persistent inflammation and immunosuppression: a common syndrome and new horizon for surgical intensive care. J Trauma Acute Care Surg. 2012; 72(6):1491-501. PMC: 3705923. DOI: 10.1097/TA.0b013e318256e000. View

Biswas S, Lopez-Collazo E . Endotoxin tolerance: new mechanisms, molecules and clinical significance. Trends Immunol. 2009; 30(10):475-87. DOI: 10.1016/j.it.2009.07.009. View

Tohira H, Jacobs I, Mountain D, Gibson N, Yeo A . Comparisons of the Outcome Prediction Performance of Injury Severity Scoring Tools Using the Abbreviated Injury Scale 90 Update 98 (AIS 98) and 2005 Update 2008 (AIS 2008). Ann Adv Automot Med. 2011; 55:255-65. PMC: 3256842. View

Morris M, Li L . Molecular mechanisms and pathological consequences of endotoxin tolerance and priming. Arch Immunol Ther Exp (Warsz). 2011; 60(1):13-8. DOI: 10.1007/s00005-011-0155-9. View

Osborn T, Tracy J, Dunne J, Pasquale M, Napolitano L . Epidemiology of sepsis in patients with traumatic injury. Crit Care Med. 2005; 32(11):2234-40. DOI: 10.1097/01.ccm.0000145586.23276.0f. View

Hoechst B, Ormandy L, Ballmaier M, Lehner F, Kruger C, Manns M . A new population of myeloid-derived suppressor cells in hepatocellular carcinoma patients induces CD4(+)CD25(+)Foxp3(+) T cells. Gastroenterology. 2008; 135(1):234-43. DOI: 10.1053/j.gastro.2008.03.020. View

Hietbrink F, Koenderman L, Althuizen M, Pillay J, Kamp V, Leenen L . Kinetics of the innate immune response after trauma: implications for the development of late onset sepsis. Shock. 2013; 40(1):21-7. DOI: 10.1097/SHK.0b013e318295a40a. View

10.

Ertel W, Kremer J, Kenney J, Steckholzer U, Jarrar D, Trentz O . Downregulation of proinflammatory cytokine release in whole blood from septic patients. Blood. 1995; 85(5):1341-7. View

11.

Hershman M, Cheadle W, Wellhausen S, Davidson P, Polk Jr H . Monocyte HLA-DR antigen expression characterizes clinical outcome in the trauma patient. Br J Surg. 1990; 77(2):204-7. DOI: 10.1002/bjs.1800770225. View

12.

Regel G, Lobenhoffer P, Grotz M, Pape H, Lehmann U, Tscherne H . Treatment results of patients with multiple trauma: an analysis of 3406 cases treated between 1972 and 1991 at a German Level I Trauma Center. J Trauma. 1995; 38(1):70-8. DOI: 10.1097/00005373-199501000-00020. View

13.

Grzelak I, Olszewski W, Zaleska M, Ziolkowska A, Durlik M, Lagiewska B . Surgical trauma evokes a rise in the frequency of hematopoietic progenitor cells and cytokine levels in blood circulation. Eur Surg Res. 1998; 30(3):198-204. DOI: 10.1159/000008577. View

14.

GREISMAN S, Young E, CAROZZA Jr F . Mechanisms of endotoxin tolerance. V. Specificity of the early and late phases of pyrogenic tolerance. J Immunol. 1969; 103(6):1223-36. View

15.

Relja B, Horstmann J, Kontradowitz K, Jurida K, Schaible A, Neunaber C . Nlrp1 inflammasome is downregulated in trauma patients. J Mol Med (Berl). 2015; 93(12):1391-400. DOI: 10.1007/s00109-015-1320-0. View

16.

Beutler B, Du X, Poltorak A . Identification of Toll-like receptor 4 (Tlr4) as the sole conduit for LPS signal transduction: genetic and evolutionary studies. J Endotoxin Res. 2001; 7(4):277-80. View

17.

Kohl J . The role of complement in danger sensing and transmission. Immunol Res. 2006; 34(2):157-76. DOI: 10.1385/IR:34:2:157. View

18.

Laudanski K, De A, Brouxhon S, Kyrkanides S, Miller-Graziano C . Abnormal PGE(2) regulation of monocyte TNF-alpha levels in trauma patients parallels development of a more macrophage-like phenotype. Shock. 2004; 22(3):204-12. DOI: 10.1097/01.shk.0000135289.62159.ad. View

19.

Lacatus M . Innate immunity in surgical patients. Chirurgia (Bucur). 2013; 108(1):18-25. View

20.

Livingston D, Anjaria D, Wu J, Hauser C, Chang V, Deitch E . Bone marrow failure following severe injury in humans. Ann Surg. 2003; 238(5):748-53. PMC: 1356155. DOI: 10.1097/01.sla.0000094441.38807.09. View