Depletion of Gangliosides Enhances Articular Cartilage Repair in Mice

Overview

Journal Sci Rep

Specialty Science

Date 2017 Mar 3

PMID 28252046

Citations 9

Authors

Masatake Matsuoka

Tomohiro Onodera

Kentaro Homan

Fumio Sasazawa

Jun-Ichi Furukawa

Daisuke Momma

Rikiya Baba

Kazutoshi Hontani

Zenta Joutoku

Shinji Matsubara

Tadashi Yamashita

Norimasa Iwasaki

Affiliations

Soon will be listed here.

Abstract

Elucidation of the healing mechanisms in damaged tissues is a critical step for establishing breakthroughs in tissue engineering. Articular cartilage is clinically one of the most successful tissues to be repaired with regenerative medicine because of its homogeneous extracellular matrix and few cell types. However, we only poorly understand cartilage repair mechanisms, and hence, regenerated cartilage remains inferior to the native tissues. Here, we show that glycosylation is an important process for hypertrophic differentiation during articular cartilage repair. GM3, which is a precursor molecule for most gangliosides, was transiently expressed in surrounding damaged tissue, and depletion of GM3 synthase enhanced cartilage repair. Gangliosides also regulated chondrocyte hypertrophy via the Indian hedgehog pathway. These results identify a novel mechanism of cartilage healing through chondrocyte hypertrophy that is regulated by glycosylation. Manipulation of gangliosides and their synthases may have beneficial effects on articular cartilage repair.

Citing Articles

CCR7 depletion alleviates bony growth imbalance following physeal injury in mice.

Kim W, Sakai Y, Matsuoka M, Hosokawa Y, Fukuda R, Homan K Sci Rep. 2024; 14(1):24891.

PMID: 39438569 PMC: 11496618. DOI: 10.1038/s41598-024-75877-1.

Depletion of b-series ganglioside prevents limb length discrepancy after growth plate injury.

Hosokawa Y, Matsuoka M, Sakai Y, Fukuda R, Matsugasaki K, Homan K BMC Musculoskelet Disord. 2024; 25(1):565.

PMID: 39033138 PMC: 11264953. DOI: 10.1186/s12891-024-07704-7.

Glycosphingolipids in Osteoarthritis and Cartilage-Regeneration Therapy: Mechanisms and Therapeutic Prospects Based on a Narrative Review of the Literature.

Homan K, Onodera T, Matsuoka M, Iwasaki N Int J Mol Sci. 2024; 25(9).

PMID: 38732111 PMC: 11084896. DOI: 10.3390/ijms25094890.

Proteomic Analyses Reveals the Mechanism of Acupotomy Intervention on the Treatment of Knee Osteoarthritis in Rabbits.

Liu J, Zeng W, Lin Q, Dai R, Lu L, Guo Z Evid Based Complement Alternat Med. 2022; 2022:5698387.

PMID: 36437834 PMC: 9691303. DOI: 10.1155/2022/5698387.

Construction and analysis of finite element model of defected articular cartilage.

Lin Y, Qin J, Zhao H, Xia C Saudi J Biol Sci. 2020; 27(1):556-560.

PMID: 31889881 PMC: 6933165. DOI: 10.1016/j.sjbs.2019.11.020.

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