Noncoding Effects of Circular RNA CCDC66 Promote Colon Cancer Growth and Metastasis

Overview

Journal Cancer Res

Specialty Oncology

Date 2017 Mar 3

PMID 28249903

Citations 367

Authors

Kuei-Yang Hsiao

Ya-Chi Lin

Sachin Kumar Gupta

Ning Chang

Laising Yen

H Sunny Sun

Shaw-Jenq Tsai

Affiliations

Soon will be listed here.

Abstract

Circular RNA (circRNA) is a class of noncoding RNA whose functions remain mostly unknown. Recent studies indicate circRNA may be involved in disease pathogenesis, but direct evidence is scarce. Here, we characterize the functional role of a novel circRNA, circCCDC66, in colorectal cancer. RNA-Seq data from matched normal and tumor colon tissue samples identified numerous circRNAs specifically elevated in cancer cells, several of which were verified by quantitative RT-PCR. CircCCDC66 expression was elevated in polyps and colon cancer and was associated with poor prognosis. Gain-of-function and loss-of-function studies in colorectal cancer cell lines demonstrated that circCCDC66 controlled multiple pathological processes, including cell proliferation, migration, invasion, and anchorage-independent growth. In-depth characterization revealed that circCCDC66 exerts its function via regulation of a subset of oncogenes, and knockdown of circCCDC66 inhibited tumor growth and cancer invasion in xenograft and orthotopic mouse models, respectively. Taken together, these findings highlight a novel oncogenic function of circRNA in cancer progression and metastasis. .

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