Extraction and Characterization of Extracellular Proteins and Their Post-Translational Modifications from Arabidopsis Thaliana Suspension Cell Cultures and Seedlings: A Critical Review

Overview

Journal Proteomes

Date 2017 Mar 2

PMID 28248235

Citations 6

Authors

Mina Ghahremani

Kyla A Stigter

William Plaxton

Affiliations

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Abstract

Proteins secreted by plant cells into the extracellular space, consisting of the cell wall, apoplastic fluid, and rhizosphere, play crucial roles during development, nutrient acquisition, and stress acclimation. However, isolating the full range of secreted proteins has proven difficult, and new strategies are constantly evolving to increase the number of proteins that can be detected and identified. In addition, the dynamic nature of the extracellular proteome presents the further challenge of identifying and characterizing the post-translational modifications (PTMs) of secreted proteins, particularly glycosylation and phosphorylation. Such PTMs are common and important regulatory modifications of proteins, playing a key role in many biological processes. This review explores the most recent methods in isolating and characterizing the plant extracellular proteome with a focus on the model plant , highlighting the current challenges yet to be overcome. Moreover, the crucial role of protein PTMs in cell wall signalling, development, and plant responses to biotic and abiotic stress is discussed.

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