Hydrogen-Rich Water Ameliorates Total Body Irradiation-Induced Hematopoietic Stem Cell Injury by Reducing Hydroxyl Radical

Overview

Journal Oxid Med Cell Longev

Publisher Wiley

Specialties Cell Biology
Endocrinology

Date 2017 Mar 1

PMID 28243358

Citations 17

Authors

Junling Zhang

Xiaolei Xue

Xiaodan Han

Yuan Li

Lu Lu

Deguan Li

Saijun Fan

Affiliations

Soon will be listed here.

Abstract

We examined whether consumption of hydrogen-rich water (HW) could ameliorate hematopoietic stem cell (HSC) injury in mice with total body irradiation (TBI). The results indicated that HW alleviated TBI-induced HSC injury with respect to cell number alteration and to the self-renewal and differentiation of HSCs. HW specifically decreased hydroxyl radical (OH) levels in the c-kit cells of 4 Gy irradiated mice. Proliferative bone marrow cells (BMCs) increased and apoptotic c-kit cells decreased in irradiated mice uptaken with HW. In addition, the mean fluorescence intensity (MFI) of -H2AX and percentage of 8-oxoguanine positive cells significantly decreased in HW-treated c-kit cells, indicating that HW can alleviate TBI-induced DNA damage and oxidative DNA damage in c-kit cells. Finally, the cell cycle (P21), cell apoptosis (BCL-XL and BAK), and oxidative stress (NRF2, HO-1, NQO1, SOD, and GPX1) proteins were significantly altered by HW in irradiated mouse c-kit cells. Collectively, the present results suggest that HW protects against TBI-induced HSC injury.

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