MiR-7 Inhibits Progression of Hepatocarcinoma by Targeting KLF-4 and Promises a Novel Diagnostic Biomarker

Overview

Journal Cancer Cell Int

Publisher Biomed Central

Date 2017 Feb 28

PMID 28239300

Citations 14

Authors

Weizhong Wu

Sanguang Liu

Yunfei Liang

Zegao Zhou

Xueqing Liu

Affiliations

Soon will be listed here.

Abstract

Background: MicroRNAs are 22-24 nt non-coding RNAs that bind to the 3' UTR of target mRNAs, thereby inducing mRNA degradation or inhibiting mRNA translation. Due to their implication in the regulation of post-transcriptional processes, the role of miRNAs in hepatocellular carcinoma (HCC) has been extensively studied. However, the function of miR-7 in HCC remains to be demonstrated.

Methods: 50 paired HCC tissues and matched peritumor tissues from patients were collected. The mRNA level of miR-7 was detected by qRT-PCR. The protein level of Kruppel-like factor 4 (KLF-4) was determined by western blot. Cell proliferation and invasive ability were measured using MTT and transwell invasion assay, respectively.

Results: We demonstrated that miR-7 was downregulated in 50 HCC tissues and the low expression of miR-7 was significantly correlate with tumour size. Moreover, overexpression of miR-7 significantly inhibited the proliferation and invasion of HCC cells. Over 100 target genes of miR-7 were predicted by Targetscan, and KLF-4 was indicated as the most promising candidate. Luciferase report assay showed that KLF-4 could be silenced by miR-7, so as to restore the impairment of cell proliferation and invasion in HCC cells.

Conclusions: In summary, we revealed a role of miR-7-KLF-4 axis in HCC cells, and the combination of both biomarkers might improve HCC diagnosis.

Citing Articles

Alterations of Krüppel-like Factor Signaling and Potential Targeted Therapy for Hepatocellular Carcinoma.

Fang R, Sha C, Xie Q, Yao D, Yao M Anticancer Agents Med Chem. 2024; 25(2):75-85.

PMID: 39313900 DOI: 10.2174/0118715206301453240910044913.

miRNA and lncRNA as potential tissue biomarkers in hepatocellular carcinoma.

Mallela V, Rajtmajerova M, Trailin A, Liska V, Hemminki K, Ambrozkiewicz F Noncoding RNA Res. 2023; 9(1):24-32.

PMID: 38075204 PMC: 10700120. DOI: 10.1016/j.ncrna.2023.10.010.

Krüppel-like Factor 13 Promotes HCC Progression by Transcriptional Regulation of HMGCS1-mediated Cholesterol Synthesis.

Chen C, Xie X, Zhao X, Zuo S, Li H J Clin Transl Hepatol. 2022; 10(6):1125-1137.

PMID: 36381108 PMC: 9634771. DOI: 10.14218/JCTH.2021.00370.

Nguyen T, Do D, Nguyen T, Nguyen T, Nguyen-Thanh T, Nguyen H PLoS One. 2022; 17(4):e0267358.

PMID: 35452485 PMC: 9032416. DOI: 10.1371/journal.pone.0267358.

miRNAs in Cancer (Review of Literature).

Smolarz B, Durczynski A, Romanowicz H, Szyllo K, Hogendorf P Int J Mol Sci. 2022; 23(5).

PMID: 35269947 PMC: 8910953. DOI: 10.3390/ijms23052805.

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