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Developmental Regulation and Identification of an Isotype Encoded by AltB, an Alpha-tubulin Locus in Physarum Polycephalum

Overview
Journal Mol Cell Biol
Specialty Cell Biology
Date 1987 Sep 1
PMID 2823127
Citations 2
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Abstract

A subcloned portion of the 5' nontranslated sequence from a Physarum alpha-tubulin cDNA is specific for a single alpha-tubulin locus, altB, of Physarum polycephalum. We find that this locus is expressed only in the plasmodium and encodes at least an alpha 1-tubulin isotype, which we have designated alpha 1B. Hybridization patterns of other subclones of this cDNA reveal two sequences for alpha-tubulin at the altB locus.

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References
1.
Krammer G, Seedorf K, Little M, Schedl T . A plasmodial alpha-tubulin cDNA from Physarum polycephalum. Nucleotide sequence and comparative analysis. J Mol Biol. 1985; 183(4):633-8. DOI: 10.1016/0022-2836(85)90176-7. View

2.
Paul E, Roobol A, FOSTER K, Gull K . Patterns of tubulin isotype synthesis and usage during mitotic spindle morphogenesis in Physarum. Cell Motil Cytoskeleton. 1987; 7(3):272-81. DOI: 10.1002/cm.970070309. View

3.
Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

4.
Kilmartin J, Wright B, Milstein C . Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line. J Cell Biol. 1982; 93(3):576-82. PMC: 2112140. DOI: 10.1083/jcb.93.3.576. View

5.
Schmitt J, Cohen B . Quantitative isolation of DNA restriction fragments from low-melting agarose by Elutip-d affinity chromatography. Anal Biochem. 1983; 133(2):462-4. DOI: 10.1016/0003-2697(83)90109-4. View