Conservation of Delta-crystallin Gene Structure Between Ducks and Chickens

Overview

Journal J Mol Evol

Specialty Biochemistry

Date 1987 Jan 1

PMID 2822941

Citations 4

Authors

J Piatigorsky

B Norman

R E Jones

Affiliations

Soon will be listed here.

Abstract

A cloned chicken delta-crystallin cDNA was used to identify two putative delta-crystallin genes in the duck by Southern blot hybridization. A DNA fragment containing most of one of these genes was isolated from a library made in bacteriophage lambda Charon 28A containing genomic DNA from 14-day-old embryonic ducks. Electron microscopy, partial gene sequencing, primer extension analysis using duck mRNA, and comparison with the well-characterized chicken delta-crystallin genes suggest that our cloned duck delta-crystallin gene, like the chicken delta-crystallin genes, is 8-10 kb long and contains 17 exons. Hybridization and sequencing data show great similarity between the homologous 5' untranslated and coding exons of the duck and chicken delta-crystallin genes. Overall, the homologous introns also appear to have approximately 30% sequence similarity, and have been subject to deletion/insertion events. Our partial characterization of duck delta-crystallin gene sequences suggests that this avian and reptilian crystallin family has been conserved during evolution, as have the other crystallin gene families that are expressed in the eye lens.

Citing Articles

Crystallin genes: specialization by changes in gene regulation may precede gene duplication.

Piatigorsky J J Struct Funct Genomics. 2003; 3(1-4):131-7.

PMID: 12836692

Gene sharing by delta-crystallin and argininosuccinate lyase.

Piatigorsky J, OBrien W, Norman B, Kalumuck K, Wistow G, Borras T Proc Natl Acad Sci U S A. 1988; 85(10):3479-83.

PMID: 3368457 PMC: 280235. DOI: 10.1073/pnas.85.10.3479.

Tau-crystallin/alpha-enolase: one gene encodes both an enzyme and a lens structural protein.

Wistow G, Lietman T, Williams L, Stapel S, de Jong W, Horwitz J J Cell Biol. 1988; 107(6 Pt 2):2729-36.

PMID: 2462567 PMC: 2115652. DOI: 10.1083/jcb.107.6.2729.

Evidence for neutral and selective processes in the recruitment of enzyme-crystallins in avian lenses.

Wistow G, Anderson A, Piatigorsky J Proc Natl Acad Sci U S A. 1990; 87(16):6277-80.

PMID: 2385592 PMC: 54516. DOI: 10.1073/pnas.87.16.6277.

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