BCG Identification by DNA Restriction Fragment Patterns

Overview

Journal J Gen Microbiol

Specialty Microbiology

Date 1987 Jun 1

PMID 2822837

Citations 12

Authors

D M Collins

G W de Lisle

Affiliations

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Abstract

Seven daughter strains of BCG were characterized by restriction fragment analysis with the enzymes BstEII, PvuII and BclI. Comparisons of fragment patterns confirmed that BCG is correctly classified as a Mycobacterium bovis variant and suggested that the Swedish strain is most closely related to the original BCG strain.

Citing Articles

Genetic identification of Mycobacterium bovis BCG by restriction fragment length polymorphism analysis of the direct-repeat region.

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Evidence for absence of the MPB64 gene in some substrains of Mycobacterium bovis BCG.

Li H, Ulstrup J, Jonassen T, Melby K, NAGAI S, HARBOE M Infect Immun. 1993; 61(5):1730-4.

PMID: 8478061 PMC: 280758. DOI: 10.1128/iai.61.5.1730-1734.1993.

DNA fingerprinting of Mycobacterium bovis strains by restriction fragment analysis and hybridization with insertion elements IS1081 and IS6110.

Collins D, Erasmuson S, Stephens D, Yates G, de Lisle G J Clin Microbiol. 1993; 31(5):1143-7.

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Population genetics of Mycobacterium tuberculosis complex in Scotland analysed by pulsed-field gel electrophoresis.

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Use of various genetic markers in differentiation of Mycobacterium bovis strains from animals and humans and for studying epidemiology of bovine tuberculosis.

van Soolingen D, de Haas P, Haagsma J, Eger T, Hermans P, Ritacco V J Clin Microbiol. 1994; 32(10):2425-33.

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