Overexpression of Dehydrin Genes in Tobacco Enhances Tolerance to Cold and Drought

Overview

Journal Front Plant Sci

Date 2017 Feb 23

PMID 28224001

Citations 43

Authors

Fei Bao

Dongliang Du

Yang An

Weiru Yang

Jia Wang

Tangren Cheng

Qixiang Zhang

Affiliations

Soon will be listed here.

Abstract

Dehydrins, known as group 2 or D-11 family late-embryogenesis-abundant (LEA) proteins, play important roles in plant growth and stress tolerance. Six dehydrin genes were previously identified from the genome of . In this study, five of them (, and ) were cloned from cold-resistant 'Beijingyudie'. Real-time RT-PCR analysis indicated that all these genes could be up-regulated by one or several treatments (ABA, SA, low temperature, high temperature, PEG, and NaCl treatments). The results of spot assay demonstrated that the expression of all these dehydrins, except PmLEA8, conferred improved osmotic and freezing-resistance to the recombinant . So four dehydrin genes, and were chosen for individual over-expression in tobacco plants. The transgenic tobacco plants showed lower relative content of malondialdehyde, relative electrolyte leakage and higher relative content of water than control plants when exposed to cold and drought stress. These results demonstrated that were involved in plant responses to cold and drought.

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