Akirin2 Promotes Slow Myosin Heavy Chain Expression by CaN/NFATc1 Signaling in Porcine Skeletal Muscle Satellite Cells

Overview

Journal Oncotarget

Specialty Oncology

Date 2017 Feb 23

PMID 28223540

Citations 3

Authors

Xiaoling Chen

Yanliu Luo

Zhiqing Huang

Guangmang Liu

Hua Zhao

Affiliations

Soon will be listed here.

Abstract

The objective of this study was to evaluate the effect of Akirin2 on slow myosin heavy chain (slow MyHC, MyHC I) gene expression and its molecular mechanisms. In this study, we showed that the protein expression of Akirin2 in pig slow oxidative Psoas major muscle is higher than that in fast glycolytic tibialis anterior muscle, suggesting that Akirin2 may play a role in myofiber typing. Knockdown of Akirin2 decreased the MyHC I expression and the calcineurin (CaN) activity, and also decreased the expressions of NFATc1 and MCIP1.4. Conversely, overexpression of Akirin2 got the opposite results. Furthermore, inhibition of CaN or knockdown of NFATc1 attenuated Akirin2 overexpression-induced upregulation of MyHC I. Together, these results demonstrate that Akirin2 promotes MyHC I expression via CaN/NFATc1 signaling pathway in porcine skeletal muscle satellite cells.

Citing Articles

Comparative Transcriptome Analysis of mRNA and miRNA during the Development of Longissimus Dorsi Muscle of Gannan Yak and Tianzhu White Yak.

Niu Y, Guo D, Wei Y, Li J, Bai Y, Liu Z Animals (Basel). 2024; 14(15).

PMID: 39123804 PMC: 11311108. DOI: 10.3390/ani14152278.

Effects of sheep slaughter age on myogenic characteristics in skeletal muscle satellite cells.

Han Y, Guo W, Su R, Zhang Y, Yang L, Borjigin G Anim Biosci. 2022; 35(4):614-623.

PMID: 34991228 PMC: 8902214. DOI: 10.5713/ab.21.0193.

Akirin proteins in development and disease: critical roles and mechanisms of action.

Bosch P, Peek S, Smolikove S, Weiner J Cell Mol Life Sci. 2020; 77(21):4237-4254.

PMID: 32361777 PMC: 7606436. DOI: 10.1007/s00018-020-03531-w.

References

Schiaffino S, Reggiani C . Fiber types in mammalian skeletal muscles. Physiol Rev. 2011; 91(4):1447-531. DOI: 10.1152/physrev.00031.2010. View

Swoap S, HUNTER R, Stevenson E, FELTON H, Kansagra N, Lang J . The calcineurin-NFAT pathway and muscle fiber-type gene expression. Am J Physiol Cell Physiol. 2000; 279(4):C915-24. DOI: 10.1152/ajpcell.2000.279.4.C915. View

Macian F . NFAT proteins: key regulators of T-cell development and function. Nat Rev Immunol. 2005; 5(6):472-84. DOI: 10.1038/nri1632. View

Raj S, Skiba G, Weremko D, Fandrejewski H, Migdal W, Borowiec F . The relationship between the chemical composition of the carcass and the fatty acid composition of intramuscular fat and backfat of several pig breeds slaughtered at different weights. Meat Sci. 2010; 86(2):324-30. DOI: 10.1016/j.meatsci.2010.04.037. View

Kubis H, Hanke N, Scheibe R, Meissner J, Gros G . Ca2+ transients activate calcineurin/NFATc1 and initiate fast-to-slow transformation in a primary skeletal muscle culture. Am J Physiol Cell Physiol. 2003; 285(1):C56-63. DOI: 10.1152/ajpcell.00377.2002. View

Lefaucheur L, Lebret B, Ecolan P, Louveau I, Damon M, Prunier A . Muscle characteristics and meat quality traits are affected by divergent selection on residual feed intake in pigs. J Anim Sci. 2010; 89(4):996-1010. DOI: 10.2527/jas.2010-3493. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Chen X, Huang Z, Jia G, Wu X, Wu C . Molecular cloning, tissue distribution, and functional analysis of porcine Akirin2. Anim Biotechnol. 2012; 23(2):124-31. DOI: 10.1080/10495398.2011.652326. View

Fenyvesi R, Racz G, Wuytack F, Zador E . The calcineurin activity and MCIP1.4 mRNA levels are increased by innervation in regenerating soleus muscle. Biochem Biophys Res Commun. 2004; 320(2):599-605. DOI: 10.1016/j.bbrc.2004.06.005. View

10.

Liu Y, Li M, Ma J, Zhang J, Zhou C, Wang T . Identification of differences in microRNA transcriptomes between porcine oxidative and glycolytic skeletal muscles. BMC Mol Biol. 2013; 14:7. PMC: 3599761. DOI: 10.1186/1471-2199-14-7. View

11.

Klont R, Brocks L, Eikelenboom G . Muscle fibre type and meat quality. Meat Sci. 2011; 49S1:S219-29. View

12.

Sasaki S, Yamada T, Sukegawa S, Miyake T, Fujita T, Morita M . Association of a single nucleotide polymorphism in akirin 2 gene with marbling in Japanese Black beef cattle. BMC Res Notes. 2009; 2:131. PMC: 2715414. DOI: 10.1186/1756-0500-2-131. View

13.

Ehlers M, Celona B, Black B . NFATc1 controls skeletal muscle fiber type and is a negative regulator of MyoD activity. Cell Rep. 2014; 8(6):1639-1648. PMC: 4180018. DOI: 10.1016/j.celrep.2014.08.035. View

14.

Tartey S, Matsushita K, Vandenbon A, Ori D, Imamura T, Mino T . Akirin2 is critical for inducing inflammatory genes by bridging IκB-ζ and the SWI/SNF complex. EMBO J. 2014; 33(20):2332-48. PMC: 4253523. DOI: 10.15252/embj.201488447. View

15.

Shen L, Lei H, Zhang S, Li X, Li M, Jiang X . Comparison of energy metabolism and meat quality among three pig breeds. Anim Sci J. 2014; 85(7):770-9. DOI: 10.1111/asj.12207. View

16.

Chin E, Olson E, Richardson J, Yang Q, Humphries C, Shelton J . A calcineurin-dependent transcriptional pathway controls skeletal muscle fiber type. Genes Dev. 1998; 12(16):2499-509. PMC: 317085. DOI: 10.1101/gad.12.16.2499. View

17.

Chen X, Zhou B, Luo Y, Huang Z, Jia G, Liu G . Tissue Distribution of Porcine FTO and Its Effect on Porcine Intramuscular Preadipocytes Proliferation and Differentiation. PLoS One. 2016; 11(3):e0151056. PMC: 4786207. DOI: 10.1371/journal.pone.0151056. View

18.

Akiyama H, Iwahana Y, Suda M, Yoshimura A, Kogai H, Nagashima A . The FBI1/Akirin2 target gene, BCAM, acts as a suppressive oncogene. PLoS One. 2013; 8(11):e78716. PMC: 3819372. DOI: 10.1371/journal.pone.0078716. View

19.

Sukegawa S, Miyake T, Ibi T, Takahagi Y, Murakami H, Morimatsu F . Multiple marker effects of single nucleotide polymorphisms in three genes, AKIRIN2, EDG1 and RPL27A, for marbling development in Japanese Black cattle. Anim Sci J. 2013; 85(3):193-7. DOI: 10.1111/asj.12108. View

20.

Kim H, Lee S, Hong M, Park S, Lee Y, Kim J . Association of a single nucleotide polymorphism in the akirin 2 gene with economically important traits in Korean native cattle. Anim Genet. 2013; 44(6):750-3. DOI: 10.1111/age.12055. View