Proteomic Analysis of Postsynaptic Protein Complexes Underlying Neuronal Plasticity

Overview

Journal ACS Chem Neurosci

Publisher American Chemical Society

Specialty Neurology

Date 2017 Feb 18

PMID 28211672

Citations 7

Authors

Anthony J Baucum 2nd

Affiliations

Soon will be listed here.

Abstract

Normal neuronal communication and synaptic plasticity at glutamatergic synapses requires dynamic regulation of postsynaptic molecules. Protein expression and protein post-translational modifications regulate protein interactions that underlie this organization. In this Review, we highlight data obtained over the last 20 years that have used qualitative and quantitative proteomics-based approaches to identify postsynaptic protein complexes. Herein, we describe how these proteomics studies have helped lay the foundation for understanding synaptic physiology and perturbations in synaptic signaling observed in different pathologies. We also describe emerging technologies that can be useful in these analyses. We focus on protein complexes associated with the highly abundant and functionally critical proteins: calcium/calmodulin-dependent protein kinase II, the N-methyl-d-aspartate, and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid glutamate receptors, and postsynaptic density protein of 95 kDa.

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References

Wang N, Chen L, Cheng N, Zhang J, Tian T, Lu W . Active calcium/calmodulin-dependent protein kinase II (CaMKII) regulates NMDA receptor mediated postischemic long-term potentiation (i-LTP) by promoting the interaction between CaMKII and NMDA receptors in ischemia. Neural Plast. 2014; 2014:827161. PMC: 3964903. DOI: 10.1155/2014/827161. View

Shishkova E, Hebert A, Coon J . Now, More Than Ever, Proteomics Needs Better Chromatography. Cell Syst. 2016; 3(4):321-324. PMC: 5448283. DOI: 10.1016/j.cels.2016.10.007. View

Brown A, Baucum A, Bass M, Colbran R . Association of protein phosphatase 1 gamma 1 with spinophilin suppresses phosphatase activity in a Parkinson disease model. J Biol Chem. 2008; 283(21):14286-94. PMC: 2386916. DOI: 10.1074/jbc.M801377200. View

McGee T, Bats C, Farrant M, Cull-Candy S . Auxiliary Subunit GSG1L Acts to Suppress Calcium-Permeable AMPA Receptor Function. J Neurosci. 2015; 35(49):16171-9. PMC: 4682783. DOI: 10.1523/JNEUROSCI.2152-15.2015. View

Lyford G, Yamagata K, Kaufmann W, Barnes C, Sanders L, Copeland N . Arc, a growth factor and activity-regulated gene, encodes a novel cytoskeleton-associated protein that is enriched in neuronal dendrites. Neuron. 1995; 14(2):433-45. DOI: 10.1016/0896-6273(95)90299-6. View

Park J, Chavez A, Mineur Y, Morimoto-Tomita M, Lutzu S, Kim K . CaMKII Phosphorylation of TARPγ-8 Is a Mediator of LTP and Learning and Memory. Neuron. 2016; 92(1):75-83. PMC: 5059846. DOI: 10.1016/j.neuron.2016.09.002. View

Oppermann F, Gnad F, Olsen J, Hornberger R, Greff Z, Keri G . Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics. 2009; 8(7):1751-64. PMC: 2709199. DOI: 10.1074/mcp.M800588-MCP200. View

Liao G, Wagner D, Hsu M, Leonard J . Evidence for direct protein kinase-C mediated modulation of N-methyl-D-aspartate receptor current. Mol Pharmacol. 2001; 59(5):960-4. DOI: 10.1124/mol.59.5.960. View

Gustin R, Shonesy B, Robinson S, Rentz T, Baucum 2nd A, Jalan-Sakrikar N . Loss of Thr286 phosphorylation disrupts synaptic CaMKIIα targeting, NMDAR activity and behavior in pre-adolescent mice. Mol Cell Neurosci. 2011; 47(4):286-92. PMC: 3149813. DOI: 10.1016/j.mcn.2011.05.006. View

10.

Gregorich Z, Ge Y . Top-down proteomics in health and disease: challenges and opportunities. Proteomics. 2014; 14(10):1195-210. PMC: 4100610. DOI: 10.1002/pmic.201300432. View

11.

Bayer K, Lebel E, McDonald G, OLeary H, Schulman H, De Koninck P . Transition from reversible to persistent binding of CaMKII to postsynaptic sites and NR2B. J Neurosci. 2006; 26(4):1164-74. PMC: 2890238. DOI: 10.1523/JNEUROSCI.3116-05.2006. View

12.

Suzuki T, Okumura-Noji K, Tanaka R, Tada T . Rapid translocation of cytosolic Ca2+/calmodulin-dependent protein kinase II into postsynaptic density after decapitation. J Neurochem. 1994; 63(4):1529-37. DOI: 10.1046/j.1471-4159.1994.63041529.x. View

13.

Bruneau E, Esteban J, Akaaboune M . Receptor-associated proteins and synaptic plasticity. FASEB J. 2008; 23(3):679-88. PMC: 2653980. DOI: 10.1096/fj.08-107946. View

14.

Miller S, Patton B, Kennedy M . Sequences of autophosphorylation sites in neuronal type II CaM kinase that control Ca2(+)-independent activity. Neuron. 1988; 1(7):593-604. DOI: 10.1016/0896-6273(88)90109-2. View

15.

Yoshimura Y, Yamauchi Y, Shinkawa T, Taoka M, Donai H, Takahashi N . Molecular constituents of the postsynaptic density fraction revealed by proteomic analysis using multidimensional liquid chromatography-tandem mass spectrometry. J Neurochem. 2004; 88(3):759-68. DOI: 10.1046/j.1471-4159.2003.02136.x. View

16.

Letts V, Valenzuela A, Kirley J, Sweet H, Davisson M, Frankel W . Genetic and physical maps of the stargazer locus on mouse chromosome 15. Genomics. 1997; 43(1):62-8. DOI: 10.1006/geno.1997.4780. View

17.

Pi H, Otmakhov N, Lemelin D, De Koninck P, Lisman J . Autonomous CaMKII can promote either long-term potentiation or long-term depression, depending on the state of T305/T306 phosphorylation. J Neurosci. 2010; 30(26):8704-9. PMC: 2903435. DOI: 10.1523/JNEUROSCI.0133-10.2010. View

18.

Roux K, Kim D, Raida M, Burke B . A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. J Cell Biol. 2012; 196(6):801-10. PMC: 3308701. DOI: 10.1083/jcb.201112098. View

19.

Coultrap S, Freund R, OLeary H, Sanderson J, Roche K, DellAcqua M . Autonomous CaMKII mediates both LTP and LTD using a mechanism for differential substrate site selection. Cell Rep. 2014; 6(3):431-7. PMC: 3930569. DOI: 10.1016/j.celrep.2014.01.005. View

20.

Miller S, Kennedy M . Distinct forebrain and cerebellar isozymes of type II Ca2+/calmodulin-dependent protein kinase associate differently with the postsynaptic density fraction. J Biol Chem. 1985; 260(15):9039-46. View