Bacteriophage K20 Requires Both the OmpF Porin and Lipopolysaccharide for Receptor Function

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1987 Oct 1

PMID 2820945

Citations 16

Authors

J A Silverman

S A Benson

Affiliations

Soon will be listed here.

Abstract

Mutations which prevent absorption of the bacteriophage K20 to Escherichia coli K-12 were selected by using an altered OmpF protein which confers the ability to grow on maltodextrin in the absence of the LamB maltoporin. The mutations map in the rfa gene cluster and alter the structure of the lipopolysaccharide core.

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References

Hancock R, Reeves P . Bacteriophage resistance in Escherichia coli K-12: general pattern of resistance. J Bacteriol. 1975; 121(3):983-93. PMC: 246027. DOI: 10.1128/jb.121.3.983-993.1975. View

Wanner B . Novel regulatory mutants of the phosphate regulon in Escherichia coli K-12. J Mol Biol. 1986; 191(1):39-58. DOI: 10.1016/0022-2836(86)90421-3. View

Bachmann B . Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983; 47(2):180-230. PMC: 281571. DOI: 10.1128/mr.47.2.180-230.1983. View

CLEMENT J, Lepouce E, Marchal C, Hofnung M . Genetic study of a membrane protein: DNA sequence alterations due to 17 lamB point mutations affecting adsorption of phage lambda. EMBO J. 1983; 2(1):77-80. PMC: 555090. DOI: 10.1002/j.1460-2075.1983.tb01384.x. View

Morrison D, Leive L . Fractions of lipopolysaccharide from Escherichia coli O111:B4 prepared by two extraction procedures. J Biol Chem. 1975; 250(8):2911-19. View

Schweizer M, Hindennach I, Garten W, Henning U . Major proteins of the Escherichia coli outer cell envelope membrane. Interaction of protein II with lipopolysaccharide. Eur J Biochem. 1978; 82(1):211-7. DOI: 10.1111/j.1432-1033.1978.tb12013.x. View

Mutoh N, Furukawa H, Mizushima S . Role of lipopolysaccharide and outer membrane protein of Escherichia coli K-12 in the receptor activity for bacteriophage T4. J Bacteriol. 1978; 136(2):693-9. PMC: 218595. DOI: 10.1128/jb.136.2.693-699.1978. View

Roa M, CLEMENT J . Location of a phage binding region on an outer membrane protein. FEBS Lett. 1980; 121(1):127-9. DOI: 10.1016/0014-5793(80)81280-4. View

Beher M, Schnaitman C . Regulation of the OmpA outer membrane protein of Escherichia coli. J Bacteriol. 1981; 147(3):972-85. PMC: 216136. DOI: 10.1128/jb.147.3.972-985.1981. View

10.

Wandersman C, Schwartz M . Mutations that alter the transport function of the LamB protein in Escherichia coli. J Bacteriol. 1982; 151(1):15-21. PMC: 220177. DOI: 10.1128/jb.151.1.15-21.1982. View

11.

Schenkman S, Tsugita A, Schwartz M, Rosenbusch J . Topology of phage lambda receptor protein. Mapping targets of proteolytic cleavage in relation to binding sites for phage or monoclonal antibodies. J Biol Chem. 1984; 259(12):7570-6. View

12.

Morona R, Klose M, Henning U . Escherichia coli K-12 outer membrane protein (OmpA) as a bacteriophage receptor: analysis of mutant genes expressing altered proteins. J Bacteriol. 1984; 159(2):570-8. PMC: 215681. DOI: 10.1128/jb.159.2.570-578.1984. View

13.

Morona R, Henning U . Host range mutants of bacteriophage Ox2 can use two different outer membrane proteins of Escherichia coli K-12 as receptors. J Bacteriol. 1984; 159(2):579-82. PMC: 215682. DOI: 10.1128/jb.159.2.579-582.1984. View

14.

Benson S, DeCloux A . Isolation and characterization of outer membrane permeability mutants in Escherichia coli K-12. J Bacteriol. 1985; 161(1):361-7. PMC: 214880. DOI: 10.1128/jb.161.1.361-367.1985. View

15.

Coleman Jr W, Deshpande K . New cysE-pyrE-linked rfa mutation in Escherichia coli K-12 that results in a heptoseless lipopolysaccharide. J Bacteriol. 1985; 161(3):1209-14. PMC: 215028. DOI: 10.1128/jb.161.3.1209-1214.1985. View

16.

Tommassen J, van der Ley P, van Zeijl M, Agterberg M . Localization of functional domains in E. coli K-12 outer membrane porins. EMBO J. 1985; 4(6):1583-7. PMC: 554385. DOI: 10.1002/j.1460-2075.1985.tb03820.x. View

17.

Benz R . Porin from bacterial and mitochondrial outer membranes. CRC Crit Rev Biochem. 1985; 19(2):145-90. DOI: 10.3109/10409238509082542. View

18.

Hitchcock P, Brown T . Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983; 154(1):269-77. PMC: 217456. DOI: 10.1128/jb.154.1.269-277.1983. View