DnaA, an Essential Host Gene, and Tn5 Transposition

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1987 Oct 1

PMID 2820938

Citations 21

Authors

J C Yin

W S Reznikoff

Affiliations

Soon will be listed here.

Abstract

Mutations in dnaA, an essential gene in Escherichia coli, decrease the frequency of transposition of Tn5. An insertion mutation in the dnaA gene does not affect Tn5 gene expression. Therefore, the DnaA protein plays a role either in the transposition reaction itself or in some type of cellular regulation of transposition. Analysis of a mutation in the DnaA box, found at the outside end of IS50, is consistent with a direct interaction of the protein through these bases. IS50 transposition, which utilizes only one end containing a DnaA box, is not affected by dnaA mutations. Overproduction of the DnaA protein does not increase transposition frequencies in wild-type cells, even when the transposase is also overproduced.

Citing Articles

Identification of basepairs within Tn5 termini that are critical sfor H-NS binding to the transpososome and regulation of Tn5 transposition.

Whitfield C, Shilton B, Haniford D Mob DNA. 2012; 3:7.

PMID: 22503096 PMC: 3347997. DOI: 10.1186/1759-8753-3-7.

Genetic evidence that GTP is required for transposition of IS903 and Tn552 in Escherichia coli.

Coros A, Twiss E, Tavakoli N, Derbyshire K J Bacteriol. 2005; 187(13):4598-606.

PMID: 15968071 PMC: 1151752. DOI: 10.1128/JB.187.13.4598-4606.2005.

Escherichia coli DNA topoisomerase I copurifies with Tn5 transposase, and Tn5 transposase inhibits topoisomerase I.

Yigit H, Reznikoff W J Bacteriol. 1999; 181(10):3185-92.

PMID: 10322021 PMC: 93775. DOI: 10.1128/JB.181.10.3185-3192.1999.

Escherichia coli DNA topoisomerase I and suppression of killing by Tn5 transposase overproduction: topoisomerase I modulates Tn5 transposition.

Yigit H, Reznikoff W J Bacteriol. 1998; 180(22):5866-74.

PMID: 9811643 PMC: 107659. DOI: 10.1128/JB.180.22.5866-5874.1998.

Insertion sequences.

Mahillon J, Chandler M Microbiol Mol Biol Rev. 1998; 62(3):725-74.

PMID: 9729608 PMC: 98933. DOI: 10.1128/MMBR.62.3.725-774.1998.

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