Increased Oxidative Stress Alters Nucleosides Metabolite Levels in Sickle Cell Anemia

Overview

Journal Redox Rep

Specialties Biochemistry
Biology
Endocrinology

Date 2017 Feb 18

PMID 28209096

Citations 5

Authors

Livia Gelain Castilhos

Juliana Sorraila de Oliveira

Stephen Adeniyi Adefegha

Luana Pereira Magni

Pedro Henrique Doleski

Fatima Husein Abdalla

Cinthia Melazzo de Andrade

Daniela Bitencourt Rosa Leal

Affiliations

Soon will be listed here.

Abstract

Objectives: This study was conducted to assess the markers of oxidative stress, myeloperoxidase (MPO), acetylcholinesterase (AChE) and xanthine oxidase (XO) activities as well as the levels of nucleotide metabolites in sickle cell anemia (SCA) patients.

Methods: Fifteen SCA treated patients and 30 health subjects (control group) were selected. The markers of oxidative stress (levels of reactive oxygen species (ROS), plasma proteins, carbonyl content, lipid peroxidation (TBARS), total thiols (T-SH), glutathione and catalase activity), MPO, AChE and XO activities as well as the levels of nucleotide metabolites were measured in SCA patients.

Results: ROS, thiobarbituric acid-reactive substances (TBARS) and T-SH levels as well as the activities of catalase and MPO were significantly increased while glutathione level was reduced in SCA patients. Furthermore, a significant (P < 0.001) increase in hypoxanthine level was demonstrated in SCA patients. However, the serum levels for xanthine (P < 0.01) and uric acid (P < 0.001) were decreased in SCA patients. A significant (P < 0.001) decrease in XO activity was detected in SCA patients.

Discussion: The altered parameters in SCA patients suggest that the generation and impairment of oxidative stress in this disease as well as antioxidant markers are contributory factors towards cellular redox homeostasis and alteration of purine metabolites.

Citing Articles

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