TRIM14 Regulates Cell Proliferation and Invasion in Osteosarcoma Via Promotion of the AKT Signaling Pathway

Overview

Journal Sci Rep

Specialty Science

Date 2017 Feb 17

PMID 28205534

Citations 40

Authors

Guoxing Xu

Yongfei Guo

Dabo Xu

Yi Wang

Yafeng Shen

Feifei Wang

Yuanyuan Lv

Fanglong Song

Dawei Jiang

Yinquan Zhang

Yi Lou

Yake Meng

Yongji Yang

Yifan Kang

Affiliations

Soon will be listed here.

Abstract

Recent studies have shown that some members of the tripartite motif-containing protein (TRIM) family serve as important regulators of tumorigenesis. However, the biological role of TRIM14 in osteosarcoma remains to be established. In this study, we showed that TRIM14 is upregulated in human osteosarcoma specimens and cell lines, and correlated with osteosarcoma progression and shorter patient survival times. Functional studies demonstrated that overexpression of TRIM14 enhances osteosarcoma cell proliferation, clone formation, cell cycle procession, migration and invasion in vitro and promotes tumor growth in vivo, and conversely, its silencing has the opposite effects. Furthermore, TRIM14 overexpression induced activation of the AKT pathway. Inhibition of AKT expression reversed the TRIM14-mediated promotory effects on cell growth and mobility, in addition to TRIM14-induced epithelial-to-mesenchymal transition (EMT) and cyclin D1 upregulation. Our findings collectively suggest that TRIM14 functions as an oncogene by upregulating the AKT signaling pathway in osteosarcoma cells, supporting its potential utility as a therapeutic target for this disease.

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