Arginine Transport in Streptococcus Lactis is Catalyzed by a Cationic Exchanger

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1987 Sep 1

PMID 2819865

Citations 37

Authors

A J Driessen

B Poolman

R Kiewiet

W Konings

Affiliations

Soon will be listed here.

Abstract

Streptococcus lactis metabolizes arginine via the arginine deiminase pathway to ornithine, CO2, NH3, and ATP. The translocation of arginine and ornithine has been studied using membrane vesicles of galactose/arginine-grown cells of S. lactis fused with cytochrome c oxidase proteoliposomes by the freeze/thaw--sonication procedure earlier described. In the presence of reduced cytochrome c the fused membranes rapidly accumulate ornithine. Addition of arginine releases accumulated ornithine. Rapid uncoupler-insensitive exchange between external arginine and internal ornithine is seen at rates that are at least 60-fold higher than the rate of protonmotive force-driven arginine translocation. This arginine:ornithine exchange activity was reconstituted in proteoliposomes after solubilization of S. lactis membranes with octyl beta-D-glucopyranoside. These proteoliposomes catalyze a one-to-one exchange between arginine and ornithine. The arginine:ornithine exchange system is the first exchange system for cationic metabolites found in bacteria. Translocation of arginine via this system does not require metabolic energy obtained by arginine metabolism.

Citing Articles

Enzymology of the pathway for ATP production by arginine breakdown.

Pols T, Singh S, Deelman-Driessen C, Gaastra B, Poolman B FEBS J. 2020; 288(1):293-309.

PMID: 32306469 PMC: 7818446. DOI: 10.1111/febs.15337.

A synthetic metabolic network for physicochemical homeostasis.

Pols T, Sikkema H, Gaastra B, Frallicciardi J, Smigiel W, Singh S Nat Commun. 2019; 10(1):4239.

PMID: 31534136 PMC: 6751199. DOI: 10.1038/s41467-019-12287-2.

Arginine and Citrulline Catabolic Pathways Encoded by the Gene Cluster of Lactobacillus brevis ATCC 367.

Majsnerowska M, Noens E, Lolkema J J Bacteriol. 2018; 200(14).

PMID: 29712874 PMC: 6018368. DOI: 10.1128/JB.00182-18.

Convergent evolution of the arginine deiminase pathway: the ArcD and ArcE arginine/ornithine exchangers.

Noens E, Lolkema J Microbiologyopen. 2016; 6(1).

PMID: 27804281 PMC: 5300872. DOI: 10.1002/mbo3.412.

ArcD1 and ArcD2 Arginine/Ornithine Exchangers Encoded in the Arginine Deiminase Pathway Gene Cluster of Lactococcus lactis.

Noens E, Kaczmarek M, Zygo M, Lolkema J J Bacteriol. 2015; 197(22):3545-53.

PMID: 26324452 PMC: 4621085. DOI: 10.1128/JB.00526-15.

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