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Intracellular Assessment of ATP Levels in

Overview
Journal Bio Protoc
Specialty Biology
Date 2017 Feb 15
PMID 28194429
Citations 21
Authors
Konstantinos Palikaras
Nektarios Tavernarakis
Affiliations
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Abstract

Eukaryotic cells heavily depend on adenosine triphosphate (ATP) generated by oxidative phosphorylation (OXPHOS) within mitochondria. ATP is the major energy currency molecule, which fuels cell to carry out numerous processes, including growth, differentiation, transportation and cell death among others (Khakh and Burnstock, 2009). Therefore, ATP levels can serve as a metabolic gauge for cellular homeostasis and survival (Artal-Sanz and Tavernarakis, 2009; Gomes , 2011; Palikaras , 2015). In this protocol, we describe a method for the determination of intracellular ATP levels using a bioluminescence approach in the nematode

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