Arachidonic Acid Triggers [Ca2+]i Increases in Rat Round Spermatids by a Likely GPR Activation, ERK Signalling and ER/acidic Compartments Ca2+ Release

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2017 Feb 14

PMID 28192519

Citations 8

Authors

Joaquin Paillamanque

Ana Sanchez-Tusie

Emerson M Carmona

Claudia L Trevino

Carolina Sandoval

Francisco Nualart

Nelson Osses

Juan G Reyes

Affiliations

Soon will be listed here.

Abstract

Arachidonic acid (AA), a compound secreted by Sertoli cells (SC) in a FSH-dependent manner, is able to induce the release of Ca2+ from internal stores in round spermatids and pachytene spermatocytes. In this study, the possible site(s) of action of AA in round spermatids, the signalling pathways associated and the intracellular Ca2+ stores targeted by AA-induced signalling were pharmacologically characterized by measuring intracellular Ca2+ using fluorescent Ca2+ probes. Our results suggest that AA acts by interacting with a fatty acid G protein coupled receptor, initiating a G protein signalling cascade that may involve PLA2 and ERK activation, which in turn opens intracellular ryanodine-sensitive channels as well as NAADP-sensitive channels in acidic intracellular Ca2+ stores. The results presented here also suggest that AMPK and PKA modulate this AA-induced Ca2+ release from intracellular Ca2+ stores in round spermatids. We propose that unsaturated free fatty acid lipid signalling in the seminiferous tubule is a novel regulatory component of rat spermatogenesis.

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