Isolation of Pseudomonas Fluorescens Species Highly Resistant to Pentachlorobenzene

Overview

Journal Folia Microbiol (Praha)

Publisher Springer

Specialty Microbiology

Date 2017 Feb 12

PMID 28188482

Authors

Itxaso Montanchez

Anna Chao Kaberdina

Elena Sevillano

Lucia Gallego

Susana Rodriguez-Couto

Vladimir R Kaberdin

Affiliations

Soon will be listed here.

Abstract

Polychlorinated aromatic compounds, including pentachlorobenzenes and hexachlorobenzenes, are recalcitrant industrial pollutants that cause adverse effects on living cells. In this paper, the isolation of Pseudomonas fluorescens species with high resistance to pentachlorobenzene (PeCB) is reported. It was found that, in contrast to its slightly negative effect on P. fluorescens growth, PeCB readily inhibited the cell growth of Serratia spp. and Escherichia coli strains, thus indicating that inhibition of bacterial growth by PeCB is species-dependent. Analysis of a P. fluorescens isolate revealed that the exposure to PeCB induced production of reactive oxygen species and led to an increase in the level of alkyl hydroperoxide reductase C (AhpC), an important enzyme enhancing the cell tolerance to organic hydroperoxides usually accumulated under oxidative stress. The putative mechanism conferring PeCB resistance to P. fluorescens and the potential use of P. fluorescens in bioremediation are discussed.

References

Tartaglia L, Storz G, Brodsky M, Lai A, Ames B . Alkyl hydroperoxide reductase from Salmonella typhimurium. Sequence and homology to thioredoxin reductase and other flavoprotein disulfide oxidoreductases. J Biol Chem. 1990; 265(18):10535-40. View

Joux F, Lebaron P . Ecological implications of an improved direct viable count method for aquatic bacteria. Appl Environ Microbiol. 2006; 63(9):3643-7. PMC: 1389250. DOI: 10.1128/aem.63.9.3643-3647.1997. View

Singh S, Kang S, Mulchandani A, Chen W . Bioremediation: environmental clean-up through pathway engineering. Curr Opin Biotechnol. 2008; 19(5):437-44. DOI: 10.1016/j.copbio.2008.07.012. View

Chen X, Christopher A, Jones J, Bell S, Guo Q, Xu F . Crystal structure of the F87W/Y96F/V247L mutant of cytochrome P-450cam with 1,3,5-trichlorobenzene bound and further protein engineering for the oxidation of pentachlorobenzene and hexachlorobenzene. J Biol Chem. 2002; 277(40):37519-26. DOI: 10.1074/jbc.M203762200. View

Jacobson F, Morgan R, Christman M, Ames B . An alkyl hydroperoxide reductase from Salmonella typhimurium involved in the defense of DNA against oxidative damage. Purification and properties. J Biol Chem. 1989; 264(3):1488-96. View

Gregoraszczuk E, Ptak A, Karpeta A, Fiedor E, Wrobel A, Milewicz T . Hexachlorobenzene and pentachlorobenzene accumulation, metabolism and effect on steroid secretion and on CYP11A1 and CYP19 expression in cultured human placental tissue. Reprod Toxicol. 2013; 43:102-10. DOI: 10.1016/j.reprotox.2013.12.004. View

Mao D, Zhou Q, Chen C, Quan Z . Coverage evaluation of universal bacterial primers using the metagenomic datasets. BMC Microbiol. 2012; 12:66. PMC: 3445835. DOI: 10.1186/1471-2180-12-66. View

Bennasar A, Mulet M, Lalucat J, Garcia-Valdes E . PseudoMLSA: a database for multigenic sequence analysis of Pseudomonas species. BMC Microbiol. 2010; 10:118. PMC: 2873489. DOI: 10.1186/1471-2180-10-118. View

Poole L, ELLIS H . Flavin-dependent alkyl hydroperoxide reductase from Salmonella typhimurium. 1. Purification and enzymatic activities of overexpressed AhpF and AhpC proteins. Biochemistry. 1996; 35(1):56-64. DOI: 10.1021/bi951887s. View

10.

Robinson P, Mack G, Remmers J, Levy R, Mohadjer L . Trends of PCB, hexachlorobenzene, and beta-benzene hexachloride levels in the adipose tissue of the U.S. population. Environ Res. 1990; 53(2):175-92. DOI: 10.1016/s0013-9351(05)80118-5. View

11.

Matthiesen R, Trelle M, Hojrup P, Bunkenborg J, Jensen O . VEMS 3.0: algorithms and computational tools for tandem mass spectrometry based identification of post-translational modifications in proteins. J Proteome Res. 2005; 4(6):2338-47. DOI: 10.1021/pr050264q. View

12.

Chavarria M, Nikel P, Perez-Pantoja D, de Lorenzo V . The Entner-Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress. Environ Microbiol. 2013; 15(6):1772-85. DOI: 10.1111/1462-2920.12069. View

13.

Seaver L, Imlay J . Alkyl hydroperoxide reductase is the primary scavenger of endogenous hydrogen peroxide in Escherichia coli. J Bacteriol. 2001; 183(24):7173-81. PMC: 95566. DOI: 10.1128/JB.183.24.7173-7181.2001. View

14.

Thomas R, Gustafson D, Pott W, LONG M, Benjamin S, Yang R . Evidence for hepatocarcinogenic activity of pentachlorobenzene with intralobular variation in foci incidence. Carcinogenesis. 1998; 19(10):1855-62. DOI: 10.1093/carcin/19.10.1855. View

15.

Winn L . Homologous recombination initiated by benzene metabolites: a potential role of oxidative stress. Toxicol Sci. 2003; 72(1):143-9. DOI: 10.1093/toxsci/kfg008. View

16.

Netuschil L, Auschill T, Sculean A, Arweiler N . Confusion over live/dead stainings for the detection of vital microorganisms in oral biofilms--which stain is suitable?. BMC Oral Health. 2014; 14:2. PMC: 3898065. DOI: 10.1186/1472-6831-14-2. View

17.

Imlay J . The molecular mechanisms and physiological consequences of oxidative stress: lessons from a model bacterium. Nat Rev Microbiol. 2013; 11(7):443-54. PMC: 4018742. DOI: 10.1038/nrmicro3032. View

18.

Field J, Sierra-Alvarez R . Microbial degradation of chlorinated benzenes. Biodegradation. 2007; 19(4):463-80. DOI: 10.1007/s10532-007-9155-1. View

19.

de Bont J, Vorage M, Hartmans S, van den Tweel W . Microbial degradation of 1,3-dichlorobenzene. Appl Environ Microbiol. 1986; 52(4):677-80. PMC: 239096. DOI: 10.1128/aem.52.4.677-680.1986. View