Mass Spectrometry Captures Structural Intermediates in Protein Fiber Self-assembly

Overview

Journal Chem Commun (Camb)

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2017 Feb 11

PMID 28184384

Citations 11

Authors

Michael Landreh

Marlene Andersson

Erik G Marklund

Qiupin Jia

Qing Meng

Jan Johansson

Carol V Robinson

Anna Rising

Affiliations

Soon will be listed here.

Abstract

Self-assembling proteins, the basis for a broad range of biological scaffolds, are challenging to study using most structural biology approaches. Here we show that mass spectrometry (MS) in combination with MD simulations captures structural features of short-lived oligomeric intermediates in spider silk formation, providing direct insights into its complex assembly process.

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