Overexpression of SKP2 Inhibits the Radiation-Induced Bystander Effects of Esophageal Carcinoma

Overview

Journal Int J Environ Res Public Health

Publisher MDPI

Specialties Environmental Health
Public Health

Date 2017 Feb 9

PMID 28178195

Citations 1

Authors

Xiao-Chun Wang

Tie-Jun Zhang

Zi-Jian Guo

Chang-Yan Xiao

Xiao-Wen Ding

Fang Fang

Wen-Tao Sheng

Xu Shu

Jue Li

Affiliations

Soon will be listed here.

Abstract

Background: To investigate the effects of S-phase kinase protein 2 (SKP2) expression on the radiation induced bystander effect (RIBE) in esophageal cancer (EC) cells.

Materials And Methods: Western blot was used to detect the levels of SKP2, Rad51, and Ku70 in EC cells. Positive transfection, RNAi, micronucleus (MN), and γ-H2AX focus formation assay were used to investigate the effects of SKP2 on RIBE induced by irradiated cells.

Results: We found a significant negative correlation between SKP2 expression and MN frequency ( < 0.05) induced by RIBE. The results were further confirmed by positive transfection, RNAi, and rescue experiments.γ-H2AX focus formation assay results indicated that overexpression of SKP2 in the irradiated cells inhibited the DNA damage of RIBE cells. However, when SKP2 expression decreased in irradiated cells, the DNA damage of RIBE cells increased. Increased or decreased expression levels of SKP2 had effects on Rad51 expression under the conditions of RIBE.

Conclusions: These results showed, for the first time, that SKP2 expression can inhibit RIBE of EC cells. The mechanism may function, at least partly, through the regulation of Rad51 in the ability to repair DNA damage.

Citing Articles

MiR-339 depresses cell proliferation via directly targeting S-phase kinase-associated protein 2 mRNA in lung cancer.

Ren H, Zhang Y, Zhu H Thorac Cancer. 2018; 9(3):408-414.

PMID: 29377618 PMC: 5832474. DOI: 10.1111/1759-7714.12597.

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