An Immunohistochemical Study of Neuropeptides and Neuronal Cytoskeletal Proteins in the Neuroepithelial Component of a Spontaneous Murine Ovarian Teratoma. Primitive Neuroepithelium Displays Immunoreactivity for Neuropeptides and Neuron-associated...

Overview

Journal Am J Pathol

Publisher Elsevier

Specialty Pathology

Date 1989 Nov 1

PMID 2817080

Citations 15

Authors

D V Caccamo

M M Herman

A Frankfurter

C D Katsetos

V P Collins

L J Rubinstein

Affiliations

Soon will be listed here.

Abstract

Approximately one third of the female mice of the LTXBO strain develop spontaneous ovarian teratomas. These tumors contain a large neuroepithelial component, which includes primitive neural structures resembling embryonic neural tubes (medulloepithelial rosettes), ependymoblastic and ependymal rosettes, neuroblasts, mature ganglionic neurons, myelinated neurites, and astrocytes. The purpose of this study was to characterize these tumors according to the immunohistochemical location of some well-characterized trophic and regulatory neuropeptides and neurotransmitters, several neuronal-associated cytoskeletal proteins, and other proteins indicative of neuronal and glial differentiation. Medulloepithelial rosettes showed focal serotonin-like, opioid peptide-like and gamma-amino butyric acid-like immunoreactivity, and displayed immunostaining for the neuron-associated class III beta-tubulin isotype. The mature ganglion cells were also immunoreactive for these markers, and, in addition, for somatostatin, cholecystokinin, bombesin, glucagon, vasoactive intestinal peptide, and neuropeptide Y. Mature ganglion cells were also immunoreactive for proteins associated with the neuronal cytoskeleton (including microtubule-associated proteins, MAP2 and tau, and higher molecular weight phosphorylated and non-phosphorylated neurofilament subunits), neuron-specific enolase, and synaptophysin. Undifferentiated stem cells, ependymoblastic and ependymal rosettes, and astroglia all stained with a monoclonal antibody that recognizes all mammalian beta-tubulin isotypes, but did not react with antibodies to neuronal-associated cytoskeletal proteins or neuropeptides. Neuropeptide-like immunoreactivity and demonstration of the class III beta-tubulin isotype indicate early neuronal commitment in neoplastic primitive neuroepithelium. These patterns of immunoreactivity closely follow those encountered in the normal neurocytogenesis of the mammalian and avian forebrain, and increase the precision with which the early stages of progressive neuroepithelial differentiation can be analyzed in human embryonal tumors of the CNS.

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References

Sasaki A, Hirato J, Nakazato Y, Ishida Y . Immunohistochemical study of the early human fetal brain. Acta Neuropathol. 1988; 76(2):128-34. DOI: 10.1007/BF00688096. View

Burgoyne R, Lewis S, Sarkar S, Cowan N . Differential distribution of beta-tubulin isotypes in cerebellum. EMBO J. 1988; 7(8):2311-9. PMC: 457095. DOI: 10.1002/j.1460-2075.1988.tb03074.x. View

Fischer I, Shea T, Sapirstein V, Kosik K . Expression and distribution of microtubule-associated protein 2 (MAP2) in neuroblastoma and primary neuronal cells. Brain Res. 1986; 390(1):99-109. DOI: 10.1016/0165-3806(86)90156-2. View

Stevens L . The development of transplantable teratocarcinomas from intratesticular grafts of pre- and postimplantation mouse embryos. Dev Biol. 1970; 21(3):364-82. DOI: 10.1016/0012-1606(70)90130-2. View

Caceres A, Banker G, Binder L . Immunocytochemical localization of tubulin and microtubule-associated protein 2 during the development of hippocampal neurons in culture. J Neurosci. 1986; 6(3):714-22. PMC: 6568475. View

Artlieb U, Krepler R, Wiche G . Expression of microtubule-associated proteins, MAP-1 and MAP-2, in human neuroblastomas and differential diagnosis of immature neuroblasts. Lab Invest. 1985; 53(6):684-91. View

Pannese E . The histogenesis of the spinal ganglia. Adv Anat Embryol Cell Biol. 1974; 47(5):7-97. DOI: 10.1007/978-3-662-10338-8_2. View

Shiosaka S, Tohyama M . Immunohistochemical techniques. Prog Brain Res. 1986; 66:3-32. View

Pappas C . CNS myelin and synapses in a spontaneous mouse ovarian teratoma showing neural differentiation. An immunohistochemical and electron microscopic study. J Neuropathol Exp Neurol. 1981; 40(3):289-97. DOI: 10.1097/00005072-198105000-00007. View

10.

Herman M, Sipe J, Rubinstein L, Vandenberg S, Spence A, VRAA-JENSEN J . An experimental mouse testicular teratoma as a model for neuroepithelial neoplasia and differentiation. II. Electron microscopy. Am J Pathol. 1975; 81(2):421-44. PMC: 2032223. View

11.

Katsetos C, Herman M, Frankfurter A, Gass P, Collins V, Walker C . Cerebellar desmoplastic medulloblastomas. A further immunohistochemical characterization of the reticulin-free pale islands. Arch Pathol Lab Med. 1989; 113(9):1019-29. View

12.

Caceres A, Binder L, Payne M, Bender P, Rebhun L, Steward O . Differential subcellular localization of tubulin and the microtubule-associated protein MAP2 in brain tissue as revealed by immunocytochemistry with monoclonal hybridoma antibodies. J Neurosci. 1984; 4(2):394-410. PMC: 6564908. View

13.

Miettinen M . Synaptophysin and neurofilament proteins as markers for neuroendocrine tumors. Arch Pathol Lab Med. 1987; 111(9):813-8. View

14.

McDowell J, Kitchen I . Development of opioid systems: peptides, receptors and pharmacology. Brain Res. 1987; 434(4):397-421. DOI: 10.1016/0165-0173(87)90006-3. View

15.

Sullivan K . Structure and utilization of tubulin isotypes. Annu Rev Cell Biol. 1988; 4:687-716. DOI: 10.1146/annurev.cb.04.110188.003351. View

16.

McDonald J, Parnavelas J, Karamanlidis A, Brecha N . The morphology and distribution of peptide-containing neurons in the adult and developing visual cortex of the rat. II. Vasoactive intestinal polypeptide. J Neurocytol. 1982; 11(5):825-37. DOI: 10.1007/BF01153521. View

17.

Rubinstein L . Diagnostic markers in human neurooncology. A progress report. Ann N Y Acad Sci. 1988; 540:78-90. DOI: 10.1111/j.1749-6632.1988.tb27053.x. View

18.

McDonald J, Parnavelas J, Karamanlidis A, ROSENQUIST G, Brecha N . The morphology and distribution of peptide-containing neurons in the adult and developing visual cortex of the rat. III. Cholecystokinin. J Neurocytol. 1982; 11(6):881-95. DOI: 10.1007/BF01148306. View

19.

Dobersen M, Hammer J, Noronha A, MacIntosh T, Trapp B, Brady R . Generation and characterization of mouse monoclonal antibodies to the myelin-associated glycoprotein (MAG). Neurochem Res. 1985; 10(4):499-513. DOI: 10.1007/BF00964654. View

20.

MATUS A, Bernhardt R . High molecular weight microtubule-associated proteins are preferentially associated with dendritic microtubules in brain. Proc Natl Acad Sci U S A. 1981; 78(5):3010-4. PMC: 319489. DOI: 10.1073/pnas.78.5.3010. View